Bidirectional regulation of hippocampal mossy fiber filopodial motility by kainate receptors: A two-step model of synaptogenesis

Ayumu Tashiro, Anna Dunaevsky, Richard Blazeski, Carol A. Mason, Rafael Yuste

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The rapid motility of axonal filopodia and dendritic spines is prevalent throughout the developing CNS, although the function of this motility remains controversial. Using two-photon microscopy, we imaged hippocampal mossy fiber axons in slice cultures and discovered that filopodial extensions are highly motile. Axonal filopodial motility is actin based and is downregulated with development, although it remains in mature cultures. This motility is correlated with free extracellular space yet is inversely correlated with contact with postsynaptic targets, indicating a potential role in synaptogenesis. Filopodial motility is differentially regulated by kainate receptors: synaptic stimulation of kainate receptors enhances motility in younger slices, but it inhibits it in mature slices. We propose that neuronal activity controls filopodial motility in a developmentally regulated manner, in order to establish synaptic contacts in a two-step process. A two-step model of synaptogenesis can also explain the opposite effects of neuronal activity on the motility of dendritic protrusions.

Original languageEnglish (US)
Pages (from-to)773-784
Number of pages12
JournalNeuron
Volume38
Issue number5
DOIs
StatePublished - Jun 5 2003

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Hippocampal Mossy Fibers
Kainic Acid Receptors
Dendritic Spines
Pseudopodia
Extracellular Space
Photons
Axons
Actins
Microscopy
Down-Regulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bidirectional regulation of hippocampal mossy fiber filopodial motility by kainate receptors : A two-step model of synaptogenesis. / Tashiro, Ayumu; Dunaevsky, Anna; Blazeski, Richard; Mason, Carol A.; Yuste, Rafael.

In: Neuron, Vol. 38, No. 5, 05.06.2003, p. 773-784.

Research output: Contribution to journalArticle

Tashiro, Ayumu ; Dunaevsky, Anna ; Blazeski, Richard ; Mason, Carol A. ; Yuste, Rafael. / Bidirectional regulation of hippocampal mossy fiber filopodial motility by kainate receptors : A two-step model of synaptogenesis. In: Neuron. 2003 ; Vol. 38, No. 5. pp. 773-784.
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