Betaine administration corrects ethanol-induced defective VLDL secretion

Kusum Kharbanda, Sandra L. Todero, Brian W. Ward, John J. Cannella, Dean J. Tuma

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Our previous studies, demonstrating ethanol-induced alterations in phosphatidylcholine (PC) synthesis via the phosphatidylethanolamine methyltransferase (PEMT) pathway, implicated a defect in very low-density lipoprotein (VLDL) secretion in the pathogenesis of hepatic steatosis. The objective of this study was to determine whether VLDL secretion was reduced by chronic ethanol consumption and whether betaine supplementation, that restores PEMT activity and prevents the development of alcoholic steatosis, could normalize VLDL secretion. The VLDL secretion in rats fed with control, ethanol and the betaine supplemented diets was determined using Triton WR-1339 to inhibit plasma VLDL metabolism. We observed reduced VLDL production rates in chronic alcohol-fed rats compared to control animals. Supplementation of betaine in the ethanol diet increased VLDL production rate to values significantly higher than those observed in the control diet-fed rats. To conclude, chronic ethanol consumption impairs PC generation via the PEMT pathway resulting in diminished VLDL secretion which contributes to the development of hepatic steatosis. By increasing PEMT-mediated PC generation, betaine results in increased fat export from the liver and attenuates the development of alcoholic fatty liver.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalMolecular and cellular biochemistry
Volume327
Issue number1-2
DOIs
StatePublished - Feb 16 2009

Fingerprint

Betaine
VLDL Lipoproteins
Phosphatidylethanolamine N-Methyltransferase
Ethanol
Nutrition
Phosphatidylcholines
Rats
Diet
Liver
Alcoholic Fatty Liver
Metabolism
Animals
Fats
Alcohols
Plasmas
Defects

Keywords

  • Alcoholic liver disease
  • Betaine
  • Phosphatidylcholine
  • Phosphatidylethanolamine methyl transferase
  • S-adenosylhomocysteine
  • S-adenosylmethionine
  • Triglyceride
  • Very low-density lipoproteins

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Betaine administration corrects ethanol-induced defective VLDL secretion. / Kharbanda, Kusum; Todero, Sandra L.; Ward, Brian W.; Cannella, John J.; Tuma, Dean J.

In: Molecular and cellular biochemistry, Vol. 327, No. 1-2, 16.02.2009, p. 75-78.

Research output: Contribution to journalArticle

Kharbanda, Kusum ; Todero, Sandra L. ; Ward, Brian W. ; Cannella, John J. ; Tuma, Dean J. / Betaine administration corrects ethanol-induced defective VLDL secretion. In: Molecular and cellular biochemistry. 2009 ; Vol. 327, No. 1-2. pp. 75-78.
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