Abstract
The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated post-transcriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2936-2939 |
| Number of pages | 4 |
| Journal | Blood |
| Volume | 104 |
| Issue number | 9 |
| DOIs | |
| State | Published - Nov 1 2004 |
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ASJC Scopus subject areas
- Biochemistry
- Immunology
- Hematology
- Cell Biology
Cite this
BCL2 protein expression parallels its mRNA level in normal and malignant B cells. / Shen, Yulei; Iqbal, Javeed; Huang, James Z.; Zhou, Guimei; Chan, Wing C.
In: Blood, Vol. 104, No. 9, 01.11.2004, p. 2936-2939.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BCL2 protein expression parallels its mRNA level in normal and malignant B cells
AU - Shen, Yulei
AU - Iqbal, Javeed
AU - Huang, James Z.
AU - Zhou, Guimei
AU - Chan, Wing C.
PY - 2004/11/1
Y1 - 2004/11/1
N2 - The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated post-transcriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.
AB - The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated post-transcriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.
UR - http://www.scopus.com/inward/record.url?scp=7244251612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7244251612&partnerID=8YFLogxK
U2 - 10.1182/blood-2004-01-0243
DO - 10.1182/blood-2004-01-0243
M3 - Article
C2 - 15242877
AN - SCOPUS:7244251612
VL - 104
SP - 2936
EP - 2939
JO - Blood
JF - Blood
SN - 0006-4971
IS - 9
ER -