BCL2 protein expression parallels its mRNA level in normal and malignant B cells

Yulei Shen, Javeed Iqbal, James Z. Huang, Guimei Zhou, Wing C. Chan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated post-transcriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.

Original languageEnglish (US)
Pages (from-to)2936-2939
Number of pages4
JournalBlood
Volume104
Issue number9
DOIs
StatePublished - Nov 1 2004
Externally publishedYes

Fingerprint

B-Cell Lymphoma
Germinal Center
B-Lymphocytes
Cells
Messenger RNA
Polymerase chain reaction
Proteins
Microdissection
Laser Capture Microdissection
RNA-Directed DNA Polymerase
Microarrays
Lymphoproliferative Disorders
Lymphoma, Large B-Cell, Diffuse
Palatine Tonsil
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence
Real-Time Polymerase Chain Reaction
Lasers
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

BCL2 protein expression parallels its mRNA level in normal and malignant B cells. / Shen, Yulei; Iqbal, Javeed; Huang, James Z.; Zhou, Guimei; Chan, Wing C.

In: Blood, Vol. 104, No. 9, 01.11.2004, p. 2936-2939.

Research output: Contribution to journalArticle

Shen, Yulei ; Iqbal, Javeed ; Huang, James Z. ; Zhou, Guimei ; Chan, Wing C. / BCL2 protein expression parallels its mRNA level in normal and malignant B cells. In: Blood. 2004 ; Vol. 104, No. 9. pp. 2936-2939.
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