Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung

S. Buch, R. N.N. Han, J. Liu, A. Moore, J. D. Edelson, B. A. Freeman, M. Post, A. K. Tanswell

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Lungs exposed to elevated O2 concentrations suffer an initial loss of type I pneumocytes, followed by a reparative type II pneumocyte hyperplasia. We hypothesized that type II pneumocyte hyperplasia after exposure of young adult rats to 85% O2 in vivo would be temporally related to 1) an increased concentration of intrapulmonary basic fibroblast growth factor (bFGF), a potent stimulator of type II pneumocyte DNA synthesis in vitro, and 2) an upregulation of pneumocyte receptors for bFGF (FGF-R). Increased rat lung bFGF mRNA, relative to air-exposed control animals, was observed at 4 days of exposure, with no increase at days 6 and 14 of exposure. Parallel changes were observed with bFGF receptor (flg) mRNA. Nuclear runoff assays confirmed increased transcription of both bFGF and flg genes in response to 85% O2, whereas increased translation at 6 days of exposure was confirmed by protein immunoanalysis. Immunohistochemistry demonstrated a broad distribution of bFGF throughout the lung, including the alveolar epithelium, which increased after 6 and 14 days of exposure to 85% O2. Our findings are compatible with a role for bFGF in O2-mediated pneumocyte hyperplasia.

Original languageEnglish (US)
Pages (from-to)L455-L464
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number3 12-3
StatePublished - Jan 1 1995

Fingerprint

fibroblast growth factor 2
pneumocytes
Growth Factor Receptors
Alveolar Epithelial Cells
Fibroblast Growth Factor 2
growth factors
lungs
Gene Expression
gene expression
Lung
receptors
rats
hyperplasia
Hyperplasia
Fibroblast Growth Factor Receptors
Messenger RNA
young adults
translation (genetics)
immunohistochemistry
Young Adult

Keywords

  • fibroblast growth factor receptor
  • flg
  • pneumocyte hyperplasia
  • pulmonary oxygen toxicity
  • type II pneumocyte

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Buch, S., Han, R. N. N., Liu, J., Moore, A., Edelson, J. D., Freeman, B. A., ... Tanswell, A. K. (1995). Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung. American Journal of Physiology - Lung Cellular and Molecular Physiology, 268(3 12-3), L455-L464.

Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung. / Buch, S.; Han, R. N.N.; Liu, J.; Moore, A.; Edelson, J. D.; Freeman, B. A.; Post, M.; Tanswell, A. K.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 268, No. 3 12-3, 01.01.1995, p. L455-L464.

Research output: Contribution to journalArticle

Buch, S, Han, RNN, Liu, J, Moore, A, Edelson, JD, Freeman, BA, Post, M & Tanswell, AK 1995, 'Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 268, no. 3 12-3, pp. L455-L464.
Buch, S. ; Han, R. N.N. ; Liu, J. ; Moore, A. ; Edelson, J. D. ; Freeman, B. A. ; Post, M. ; Tanswell, A. K. / Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1995 ; Vol. 268, No. 3 12-3. pp. L455-L464.
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