Bacteriophage phi6

A lipid containing virus of Pseudomonas phaseolicola

A. K. Vidaver, R. K. Koski, James L Van Etten

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

The purification and properties of a lipid containing bacteriophage, phi6, are described. The phage contains a lipid envelope which is probably essential for infection. Infectivity of phi6 was lost in the presence of organic solvents, sodium deoxycholate, and phospholipase A. The fatty acid composition of the phage lipid was similar to that of the Pseudomonas phaseolicola host cells. The phage was composed of about 25% lipid, 13% RNA, and 62% protein. The buoyant density of phi6 was 1.27 g/ml in cesium chloride. The morphology of phi6 was unusual; it had a polyhedral head of about 60 nm surrounded by a membranous, compressible envelope which appeared to assume an elongated configuration upon attachment to pili. The adsorption rate constant was 3.3 x 10-10ml/min in a semi synthetic medium and 3.8 x 10-10 ml/min in a nutrient broth yeast extract medium. The latent period was shorter in the former medium (80-115 min compared with 120-160 min), and the average burst size was larger (250-400 compared with 125-150). The eclipse period coincided with the latent period.

Original languageEnglish (US)
Pages (from-to)799-805
Number of pages7
JournalJournal of virology
Volume11
Issue number5
StatePublished - Jan 1 1973

Fingerprint

Pseudomonas
bacteriophages
Bacteriophages
Viruses
Lipids
viruses
latent period
lipids
deoxycholic acid
cesium
Deoxycholic Acid
Phospholipases A
fimbriae
yeast extract
Adsorption
chlorides
adsorption
Fatty Acids
pathogenicity
Yeasts

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Bacteriophage phi6 : A lipid containing virus of Pseudomonas phaseolicola. / Vidaver, A. K.; Koski, R. K.; Van Etten, James L.

In: Journal of virology, Vol. 11, No. 5, 01.01.1973, p. 799-805.

Research output: Contribution to journalArticle

Vidaver, A. K. ; Koski, R. K. ; Van Etten, James L. / Bacteriophage phi6 : A lipid containing virus of Pseudomonas phaseolicola. In: Journal of virology. 1973 ; Vol. 11, No. 5. pp. 799-805.
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