Bacteriophage lambda-based expression vectors

Research output: Contribution to journalReview article

10 Scopus citations

Abstract

Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadvantages associated with its mode of growth and its size. Some lambda vectors have been designed to be readily converted into plasmids or phagemids, and there are a variety of promoters and fusions that can be used to drive expression of foreign genes. Screening lambda libraries with antibodies or ligands is a powerful way of identifying novel genes.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume17
Issue number3
DOIs
Publication statusPublished - Jun 28 2001

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Keywords

  • Bacteriophage lambda
  • Expression vectors
  • Fusion proteins
  • Recombinant DNA
  • Subcloning

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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