Bacterial long-chain fatty acid transport. Identification of amino acid residues within the outer membrane protein FadL required for activity

G. B. Kumar, Paul N Black

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The outer membrane protein FadL (product of the fadL gene) of Escherichia coli is required for the specific binding and transport of exogenous long- chain fatty acids prior to metabolic utilization. The carboxyl end of FadL has been proposed to play a crucial role by facilitating the transport of long-chain fatty acids. In an attempt to define specific amino acid residues within carboxyl region of FadL essential for activity, a series of deletion and point mutations within the 3' end of the fadL+ gene have been constructed and characterized. These fadL mutants were classified into three categories based on functional properties attributable to the altered FadL proteins: (i) those that had essentially wild-type levels of long-chain fatty acid binding and transport, (ii) those that had wild-type levels of long-chain fatty acid binding but were defective in transport, and (iii) those that were defective for both long-chain fatty acid binding and transport. These findings demonstrate that amino acid residues Phe448, Pro428, Val410, and Ser397 are required for optimal levels of long- chain fatty acid transport and that amino acid residues Pro428 and Val410 are essential for long-chain fatty acid binding.

Original languageEnglish (US)
Pages (from-to)15469-15476
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number21
StatePublished - Jan 1 1993

Fingerprint

Membrane Proteins
Fatty Acids
Amino Acids
Genes
Sequence Deletion
Point Mutation
Escherichia coli
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{91223952de954257a7068479848bb000,
title = "Bacterial long-chain fatty acid transport. Identification of amino acid residues within the outer membrane protein FadL required for activity",
abstract = "The outer membrane protein FadL (product of the fadL gene) of Escherichia coli is required for the specific binding and transport of exogenous long- chain fatty acids prior to metabolic utilization. The carboxyl end of FadL has been proposed to play a crucial role by facilitating the transport of long-chain fatty acids. In an attempt to define specific amino acid residues within carboxyl region of FadL essential for activity, a series of deletion and point mutations within the 3' end of the fadL+ gene have been constructed and characterized. These fadL mutants were classified into three categories based on functional properties attributable to the altered FadL proteins: (i) those that had essentially wild-type levels of long-chain fatty acid binding and transport, (ii) those that had wild-type levels of long-chain fatty acid binding but were defective in transport, and (iii) those that were defective for both long-chain fatty acid binding and transport. These findings demonstrate that amino acid residues Phe448, Pro428, Val410, and Ser397 are required for optimal levels of long- chain fatty acid transport and that amino acid residues Pro428 and Val410 are essential for long-chain fatty acid binding.",
author = "Kumar, {G. B.} and Black, {Paul N}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "268",
pages = "15469--15476",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "21",

}

TY - JOUR

T1 - Bacterial long-chain fatty acid transport. Identification of amino acid residues within the outer membrane protein FadL required for activity

AU - Kumar, G. B.

AU - Black, Paul N

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The outer membrane protein FadL (product of the fadL gene) of Escherichia coli is required for the specific binding and transport of exogenous long- chain fatty acids prior to metabolic utilization. The carboxyl end of FadL has been proposed to play a crucial role by facilitating the transport of long-chain fatty acids. In an attempt to define specific amino acid residues within carboxyl region of FadL essential for activity, a series of deletion and point mutations within the 3' end of the fadL+ gene have been constructed and characterized. These fadL mutants were classified into three categories based on functional properties attributable to the altered FadL proteins: (i) those that had essentially wild-type levels of long-chain fatty acid binding and transport, (ii) those that had wild-type levels of long-chain fatty acid binding but were defective in transport, and (iii) those that were defective for both long-chain fatty acid binding and transport. These findings demonstrate that amino acid residues Phe448, Pro428, Val410, and Ser397 are required for optimal levels of long- chain fatty acid transport and that amino acid residues Pro428 and Val410 are essential for long-chain fatty acid binding.

AB - The outer membrane protein FadL (product of the fadL gene) of Escherichia coli is required for the specific binding and transport of exogenous long- chain fatty acids prior to metabolic utilization. The carboxyl end of FadL has been proposed to play a crucial role by facilitating the transport of long-chain fatty acids. In an attempt to define specific amino acid residues within carboxyl region of FadL essential for activity, a series of deletion and point mutations within the 3' end of the fadL+ gene have been constructed and characterized. These fadL mutants were classified into three categories based on functional properties attributable to the altered FadL proteins: (i) those that had essentially wild-type levels of long-chain fatty acid binding and transport, (ii) those that had wild-type levels of long-chain fatty acid binding but were defective in transport, and (iii) those that were defective for both long-chain fatty acid binding and transport. These findings demonstrate that amino acid residues Phe448, Pro428, Val410, and Ser397 are required for optimal levels of long- chain fatty acid transport and that amino acid residues Pro428 and Val410 are essential for long-chain fatty acid binding.

UR - http://www.scopus.com/inward/record.url?scp=0027184393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027184393&partnerID=8YFLogxK

M3 - Article

VL - 268

SP - 15469

EP - 15476

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 21

ER -