Bacterial expression and in vitro folding of the β-subunit of human chorionic gonadotropin (hCGβ) and functional assembly of recombinant hCGβ with hCGα

Jeffrey R. Huth, Sheila E. Norton, Oksana Lockridge, Toshihiko Shikone, Aaron J W Hsueh, Raymond W. Ruddon

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A bacterial expression system for the β-subunit of hCG (hCGβ) has been developed to produce suitable amounts of this protein for structural and biological studies. To produce hCGβ in Escherichia coli, the nucleotide sequence that encodes the amino acid leader sequence was removed from the hCGβ complementary DNA, and the gene was cloned into a pET expression vector. After induction of protein synthesis in host bacteria, recombinant hCGβ (rhCGβ) accumulated in inclusion bodies in an unfolded state. The inclusion bodies were purified from induced cultures of E. coli, solubilized in urea, and fractionated by reverse phase HPLC. In this way, 6-7 mg unfolded hCGβ were recovered from 1 liter culture. rhCGβ was folded in the presence of 6.4 mM cysteamine and 3.6 mM cystamine at pH 8.7 at a final concentration of 0.02 mg/ml protein. The folded protein assembled with urinary hCGα and the purified rhCGβ/urinary α dimer bound to and activated the human LH/CG receptor permanently expressed in a cell line, indicating that it was a functional hormone. The rhCGβ/urinary α dimer also stimulated in vivo ovulation in rats, thus confirming the biological activity of bacterially expressed hCGβ. Because E. coli lacks the ability to glycosylate proteins, these activity results indicate that the N-linked and O-linked oligosaccharides of hCGβ are not required for protein folding, subunit assembly, or full biological activity. The success of producing hCGβ in bacteria and of folding it in vitro implies that the β-subunits of the other members of the glycoprotein hormone family, LH, FSH, and TSH, can also be produced in this manner. This may facilitate structural studies of these hormones as well as lead to the production of recombinant hormones for biological studies and clinical use.

Original languageEnglish (US)
Pages (from-to)911-918
Number of pages8
JournalEndocrinology
Volume135
Issue number3
DOIs
StatePublished - Jan 1 1994

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Chorionic Gonadotropin
Hormones
Inclusion Bodies
Escherichia coli
Proteins
Cystamine
In Vitro Techniques
Bacteria
Cysteamine
LH Receptors
Protein Folding
Protein Subunits
Ovulation
Oligosaccharides
Urea
Amino Acid Sequence
Glycoproteins
Complementary DNA
High Pressure Liquid Chromatography
Cell Line

ASJC Scopus subject areas

  • Endocrinology

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Bacterial expression and in vitro folding of the β-subunit of human chorionic gonadotropin (hCGβ) and functional assembly of recombinant hCGβ with hCGα. / Huth, Jeffrey R.; Norton, Sheila E.; Lockridge, Oksana; Shikone, Toshihiko; Hsueh, Aaron J W; Ruddon, Raymond W.

In: Endocrinology, Vol. 135, No. 3, 01.01.1994, p. 911-918.

Research output: Contribution to journalArticle

Huth, Jeffrey R. ; Norton, Sheila E. ; Lockridge, Oksana ; Shikone, Toshihiko ; Hsueh, Aaron J W ; Ruddon, Raymond W. / Bacterial expression and in vitro folding of the β-subunit of human chorionic gonadotropin (hCGβ) and functional assembly of recombinant hCGβ with hCGα. In: Endocrinology. 1994 ; Vol. 135, No. 3. pp. 911-918.
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