B7-2 regulates survival, phenotype, and function of APCs

Deepak Yadav, Nora Sarvetnick

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The absence of B7-2-mediated costimulation protects NOD mice from the development of diabetes. Although the effects of B7-2 on T cell priming are well known, its impact on the function of APCs is not fully elucidated. We tested APC function and survival in mice lacking B7-2. A significant reduction in the phagocytic ability was observed in both splenic and pancreatic lymph node-associated dendritic cells (DCs) in B7-2 knockout (KO) mice. DCs from B7-2KO mice exhibited enhanced susceptibility to death, which was reflected by their reduced total cell numbers. Phenotypic analysis of APCs in B7-2KO mice revealed a significantly decreased proportion of CD8α +CD205+ DCs. Interestingly, an enhanced proportion of B7-H1+ and B7-DC+ DCs were observed in B7-2KO mice. Lastly, we found that B7-2 deficiency significantly diminished the PKC-ε response in APCs upon CD28-Ig stimulation. In conclusion our data suggests that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival.

Original languageEnglish (US)
Pages (from-to)6236-6241
Number of pages6
JournalJournal of Immunology
Volume178
Issue number10
DOIs
StatePublished - May 15 2007

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Dendritic Cells
Phenotype
Inbred NOD Mouse
Knockout Mice
Cell Count
Lymph Nodes
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

B7-2 regulates survival, phenotype, and function of APCs. / Yadav, Deepak; Sarvetnick, Nora.

In: Journal of Immunology, Vol. 178, No. 10, 15.05.2007, p. 6236-6241.

Research output: Contribution to journalArticle

Yadav, Deepak ; Sarvetnick, Nora. / B7-2 regulates survival, phenotype, and function of APCs. In: Journal of Immunology. 2007 ; Vol. 178, No. 10. pp. 6236-6241.
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