B cell hybridoma presents both B-cell and T-cell epitopes for stimulating antibody production via CD23 pathway

Robert Schmaltz, Yan Yu Wang, Qing Xian Kung, Fu Tong Liu, Thomas M Petro, Swey Shen Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

CD23+ B cell hybridoma 17A11, pulsed with IgE:TNP-KLH triggered IgA, IgG, and IgE antibody production via CD23-mediated presentation. Prior anti- CD23 treatment abrogated 95% of the humoral antibody responses. Both B and T cell epitopes were presented by 17A11. B cell epitopes as recognized by IgG but not T cell epitopes were sensitive to treatment with 0.2 M acetic acid. Efficacy of antigen presentation via CD23 on 17A11 was comparable to that mediated via surface immunoglobulins (sIg) on a CD23 negative 4.5 parental fusion partner B cell line. This is the first demonstration that IgE:TNP-KLH pulsed B cell hybridomas present both B-and T-cell epitopes in stimulating IgA, IgG, and IgE antibody production, and raise a pertinent issue whether IgE antibodies produced under pathophysiological conditions may serve as positive feedback signal for sustaining production of different classes of antibodies.

Original languageEnglish (US)
Pages (from-to)481-493
Number of pages13
JournalImmunological Investigations
Volume25
Issue number5-6
DOIs
StatePublished - Jan 1 1996

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T-Lymphocyte Epitopes
Hybridomas
Immunoglobulin E
Antibody Formation
B-Lymphocyte Epitopes
B-Lymphocytes
Immunoglobulin G
Immunoglobulin A
B-Cell Antigen Receptors
Immunoglobulin Isotypes
Antigen Presentation
Acetic Acid
Cell Line
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

B cell hybridoma presents both B-cell and T-cell epitopes for stimulating antibody production via CD23 pathway. / Schmaltz, Robert; Wang, Yan Yu; Kung, Qing Xian; Liu, Fu Tong; Petro, Thomas M; Chen, Swey Shen.

In: Immunological Investigations, Vol. 25, No. 5-6, 01.01.1996, p. 481-493.

Research output: Contribution to journalArticle

Schmaltz, Robert ; Wang, Yan Yu ; Kung, Qing Xian ; Liu, Fu Tong ; Petro, Thomas M ; Chen, Swey Shen. / B cell hybridoma presents both B-cell and T-cell epitopes for stimulating antibody production via CD23 pathway. In: Immunological Investigations. 1996 ; Vol. 25, No. 5-6. pp. 481-493.
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