Autonomous growth of lymphoid cells following IL-2 expression from retrovirus vectors containing HIV-1 trans-acting elements

Ilya M. Shapiro, Craig Meier, Victoria Vlach, Thomas L McDonald, Hans Wigzell, Mario Stevenson

Research output: Contribution to journalArticle

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Abstract

The human immunodeficiency virus type I (HIV-1) possesses powerful regulatory elements that control the rate of replication of HIV-1 and subsequent processing of HIV-1 genes. We have used this regulatory mechanism to drive expression of foreign genes inserted in retrovirus vectors. This approach was used to express the human IL-2 gene in IL-2-dependent mouse CTLL-2 cells to determine the role of autonomous growth in maintaining proliferation of virus-infected T lymphocytes during HTLV-1-induced adult T-cell leukemia (ATL). Expression of IL-2 sequences in IL-2-dependent mouse CTLL-2 cells resulted in autonomous growth of IL-2-independent CTLL-2 clones. Endogenous expression of IL-2 appeared to interrupt normal constraints of growth in that these IL-2-independent clones showed reduced cell-density-dependent inhibition but not a tumorigenic phenotype. IL-2-independent CTLL-2 clones did not secrete detectable quantities of IL-2 into culture supernatant and exhibited reduced sensitivity to the inhibitory effects of both IL-2 and IL-2 receptor antibody. These results suggest that the IL-2 autocrine loop within these cells involves intracellular IL-2/IL-2 receptor binding. The apparent lack of IL-2 production and poor responsiveness to IL-2 or IL-2 antibodies displayed by cell lines from ATL patients may be explained by an intracellular IL-2/IL-2 receptor autocrine loop.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalSomatic Cell and Molecular Genetics
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1990

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Retroviridae
Interleukin-2
HIV-1
Lymphocytes
Growth
Interleukin-2 Receptors
Adult T Cell Leukemia Lymphoma
Clone Cells
Human T-lymphotropic virus 1
Antibodies
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Autonomous growth of lymphoid cells following IL-2 expression from retrovirus vectors containing HIV-1 trans-acting elements. / Shapiro, Ilya M.; Meier, Craig; Vlach, Victoria; McDonald, Thomas L; Wigzell, Hans; Stevenson, Mario.

In: Somatic Cell and Molecular Genetics, Vol. 16, No. 1, 01.01.1990, p. 1-14.

Research output: Contribution to journalArticle

Shapiro, Ilya M. ; Meier, Craig ; Vlach, Victoria ; McDonald, Thomas L ; Wigzell, Hans ; Stevenson, Mario. / Autonomous growth of lymphoid cells following IL-2 expression from retrovirus vectors containing HIV-1 trans-acting elements. In: Somatic Cell and Molecular Genetics. 1990 ; Vol. 16, No. 1. pp. 1-14.
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