Autocrine transforming growth factor provides a growth advantage to malignant cells by facilitating re-entry into the cell cycle from suboptimal growth states

Dianhua Jiang, Haisu Yang, James K.V. Willson, Jiurong Liang, Lisa E. Humphrey, Elizabeth Zborowska, Degeng Wang, Jason Foster, Robert Fan, Michael G. Brattain

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

CBS human colon carcinoma cells are poorly tumorigenic in athymic nude mice, whereas FET colon carcinoma cells are non-tumorigenic. Both cell lines have well differentiated properties in tissue culture. Transforming growth factor α (TGF-α) was ectopically expressed by stable transfection of a TGF- α cDNA under repressible tetracycline control. The TGF-α-transfected cells showed enhanced clonal initiation and shortened lag phase growth in tissue culture without an alteration in doubling time in exponential phase relative to untransfected cells. Furthermore, the TGF-α transfectants showed increased independence from exogenous growth factors in clonal growth assays and induction of DNA synthesis after release from quiescence. Growth factor independence was associated with sustained epidermal growth factor receptor activation in quiescent TGF-α-transfected cells and the requirement of exogenous insulin for stimulation of quiescent cells to reenter the cell cycle. Higher cloning, reduced lag time in tissue, and the acquisition of growth factor independence for DNA synthesis without a change in doubling time of TGF-α-transfected cells indicate that autocrine TGF-α functions by facilitating re-entry into the cell cycle from sub-optimal growth states rather than promoting or controlling the proliferation of actively cycling cells. The modulation of growth regulation by autocrine TGF-α was associated with increased malignant properties as TGF-α transfectants showed increased tumorigenicity in athymic nude mice. The administration of tetracycline reversed the effects of TGF-α expression in these cells both in vivo and in vitro, indicating that the alterations of the biological properties were due to the expression of TGF-α. Since these cells are continuously grown in a completely chemically defined medium without serum supplementation, it was possible to assign the mechanism underlying the generation of growth factor independence to the replacement of a requirement for exogenous insulin in parental cells by autocrine TGF-α.

Original languageEnglish (US)
Pages (from-to)31471-31479
Number of pages9
JournalJournal of Biological Chemistry
Volume273
Issue number47
DOIs
StatePublished - Nov 20 1998

Fingerprint

Reentry
Transforming Growth Factors
Cell Cycle
Cells
Growth
Nude Mice
Intercellular Signaling Peptides and Proteins
Tissue culture
Tetracycline
Colon
Insulin
Carcinoma
Cloning
DNA
Field effect transistors
Epidermal Growth Factor Receptor
Transfection
Organism Cloning
Assays
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Autocrine transforming growth factor provides a growth advantage to malignant cells by facilitating re-entry into the cell cycle from suboptimal growth states. / Jiang, Dianhua; Yang, Haisu; Willson, James K.V.; Liang, Jiurong; Humphrey, Lisa E.; Zborowska, Elizabeth; Wang, Degeng; Foster, Jason; Fan, Robert; Brattain, Michael G.

In: Journal of Biological Chemistry, Vol. 273, No. 47, 20.11.1998, p. 31471-31479.

Research output: Contribution to journalArticle

Jiang, Dianhua ; Yang, Haisu ; Willson, James K.V. ; Liang, Jiurong ; Humphrey, Lisa E. ; Zborowska, Elizabeth ; Wang, Degeng ; Foster, Jason ; Fan, Robert ; Brattain, Michael G. / Autocrine transforming growth factor provides a growth advantage to malignant cells by facilitating re-entry into the cell cycle from suboptimal growth states. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 47. pp. 31471-31479.
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abstract = "CBS human colon carcinoma cells are poorly tumorigenic in athymic nude mice, whereas FET colon carcinoma cells are non-tumorigenic. Both cell lines have well differentiated properties in tissue culture. Transforming growth factor α (TGF-α) was ectopically expressed by stable transfection of a TGF- α cDNA under repressible tetracycline control. The TGF-α-transfected cells showed enhanced clonal initiation and shortened lag phase growth in tissue culture without an alteration in doubling time in exponential phase relative to untransfected cells. Furthermore, the TGF-α transfectants showed increased independence from exogenous growth factors in clonal growth assays and induction of DNA synthesis after release from quiescence. Growth factor independence was associated with sustained epidermal growth factor receptor activation in quiescent TGF-α-transfected cells and the requirement of exogenous insulin for stimulation of quiescent cells to reenter the cell cycle. Higher cloning, reduced lag time in tissue, and the acquisition of growth factor independence for DNA synthesis without a change in doubling time of TGF-α-transfected cells indicate that autocrine TGF-α functions by facilitating re-entry into the cell cycle from sub-optimal growth states rather than promoting or controlling the proliferation of actively cycling cells. The modulation of growth regulation by autocrine TGF-α was associated with increased malignant properties as TGF-α transfectants showed increased tumorigenicity in athymic nude mice. The administration of tetracycline reversed the effects of TGF-α expression in these cells both in vivo and in vitro, indicating that the alterations of the biological properties were due to the expression of TGF-α. Since these cells are continuously grown in a completely chemically defined medium without serum supplementation, it was possible to assign the mechanism underlying the generation of growth factor independence to the replacement of a requirement for exogenous insulin in parental cells by autocrine TGF-α.",
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AU - Jiang, Dianhua

AU - Yang, Haisu

AU - Willson, James K.V.

AU - Liang, Jiurong

AU - Humphrey, Lisa E.

AU - Zborowska, Elizabeth

AU - Wang, Degeng

AU - Foster, Jason

AU - Fan, Robert

AU - Brattain, Michael G.

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