Autocrine transforming growth factor-α is associated with modulation of a 130 kD RB protein species and 116/110 Rb as revealed by an antisense TGFα expression vector

Michael G Brattain

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Specific RB protein species modulated by autocrine TGFα are unknown. HCT116 cells transfected with TGFα antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RB species. Nutrients alone (N) induced maximal mitogenesis in HCT116 NEO and modulated both 130 kD and 116 kD/110 kD RB species. TGFα antisense transfected clones required nutrients plus growth factors (GF) for maximal mitogenesis and both 130 kD/120 kD as well as 116 kD/110 kD species were modulated. Thus, autocrine TGFα (HCT116) was associated with 130 kD and 116/110 kD modulation while its suppression allowed modulation of a 120 kD species as well.

Original languageEnglish (US)
Pages (from-to)1025-1033
Number of pages9
JournalInternational Journal of Oncology
Volume8
Issue number6
StatePublished - Jun 4 1996

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Transforming Growth Factors
HCT116 Cells
Food
Intercellular Signaling Peptides and Proteins
Proteins
Clone Cells
N-(2-(mercaptomethyl)-3-phenylbutanoyl)-L-alanine

Keywords

  • RB species
  • antisense TGFα transfection
  • autocrine TGFα
  • colon cancer cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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abstract = "Specific RB protein species modulated by autocrine TGFα are unknown. HCT116 cells transfected with TGFα antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RB species. Nutrients alone (N) induced maximal mitogenesis in HCT116 NEO and modulated both 130 kD and 116 kD/110 kD RB species. TGFα antisense transfected clones required nutrients plus growth factors (GF) for maximal mitogenesis and both 130 kD/120 kD as well as 116 kD/110 kD species were modulated. Thus, autocrine TGFα (HCT116) was associated with 130 kD and 116/110 kD modulation while its suppression allowed modulation of a 120 kD species as well.",
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AB - Specific RB protein species modulated by autocrine TGFα are unknown. HCT116 cells transfected with TGFα antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RB species. Nutrients alone (N) induced maximal mitogenesis in HCT116 NEO and modulated both 130 kD and 116 kD/110 kD RB species. TGFα antisense transfected clones required nutrients plus growth factors (GF) for maximal mitogenesis and both 130 kD/120 kD as well as 116 kD/110 kD species were modulated. Thus, autocrine TGFα (HCT116) was associated with 130 kD and 116/110 kD modulation while its suppression allowed modulation of a 120 kD species as well.

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