Augmentation of oxidant injury to human pulmonary epithelial cells by the Pseudomonas aeruginosa siderophore pyochelin

Bradley E Britigan, George T. Rasmussen, Charles D. Cox

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Pseudomonas aeruginosa causes acute and chronic infections of the human lung, with resultant tissue injury. We have previously shown that iron hound to pyochelin, a siderophore secreted by the organism to acquire iron, is an efficient catalyst for hydroxyl radical (HO-) formation and augments injury to pulmonary artery endothelial cells resulting from their exposure to superoxide (O2-·) and/or H2O2. Sources for O2-· and H2O2 included phorbol myristate acetate (PMA)-stimulated neutrophils and pyocyanin. Pyocyanin, another P. aeruginosa secretory product, undergoes cell-mediated redox, thereby forming O2-· and H2O2. In P. aeruginosa lung infections, damage to airway epithelial cells is probably more extensive than that to endothelial cells. Therefore, we examined whether ferripyochelin also augments oxidant-mediated damage to airway epithelial cells. A549 cells, a human type II alveolar epithelial cell line, was exposed to H2O2, PMA- stimulated neutrophils, or pyocyanin, and injury was determined by release of 51Cr from prelabeled cells. Ferripyochelin significantly increased (>10- fold) oxidant-mediated cell injury regardless of whether H2O2, neutrophils, or pyocyanin was employed. Apo-pyochelin was not effective, and ferripyochelin was not toxic by itself at the concentrations employed. Spin trapping with α-(4-pyrridyl-1-oxide)-N-t-butyl-nitrone-ethanol confirmed the generation of HO-, and injury was decreased by a variety of antioxidants, including superoxide dismutase, catalase, and dimethylthiourea. These data are consistent with the hypothesis that the presence of ferripyochelin at sites of P. aeruginosa lung infection could contribute to tissue injury through its ability to promote HO--mediated damage to airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)1071-1076
Number of pages6
JournalInfection and Immunity
Volume65
Issue number3
StatePublished - Mar 4 1997

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Siderophores
Pyocyanine
Oxidants
Pseudomonas aeruginosa
Epithelial Cells
Lung
Wounds and Injuries
Neutrophils
Tetradecanoylphorbol Acetate
Iron
Endothelial Cells
Infection
Spin Trapping
Alveolar Epithelial Cells
Poisons
Superoxides
Hydroxyl Radical
Catalase
Oxides
Pulmonary Artery

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Augmentation of oxidant injury to human pulmonary epithelial cells by the Pseudomonas aeruginosa siderophore pyochelin. / Britigan, Bradley E; Rasmussen, George T.; Cox, Charles D.

In: Infection and Immunity, Vol. 65, No. 3, 04.03.1997, p. 1071-1076.

Research output: Contribution to journalArticle

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