ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes

Yong Ding, Ivan Ndamukong, Zaoshi Xu, Hanna Lapko, Michael E Fromm, Zoya V Avramova

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Tri-methylated H3 lysine 4 (H3K4me3) is associated with transcriptionally active genes, but its function in the transcription process is still unclear. Point mutations in the catalytic domain of ATX1 (ARABIDOPSIS TRITHORAX1), a H3K4 methyltransferase, and RNAi knockdowns of subunits of the AtCOMPASS-like (Arabidopsis Complex Proteins Associated with Set) were used to address this question. We demonstrate that both ATX1 and AtCOMPASS-like are required for high level accumulation of TBP (TATA-binding protein) and Pol II at promoters and that this requirement is independent of the catalytic histone modifying activity. However, the catalytic function is critically required for transcription as H3K4me3 levels determine the efficiency of transcription elongation. The roles of H3K4me3, ATX1, and AtCOMPASS-like may be of a general relevance for transcription of Trithorax-activated eukaryotic genes.

Original languageEnglish (US)
Article numbere1003111
JournalPLoS genetics
Volume8
Issue number12
DOIs
StatePublished - Dec 1 2012

Fingerprint

Arabidopsis
transcription (genetics)
protein
gene
Arabidopsis Proteins
TATA-Box Binding Protein
Genes
mutation
genes
Methyltransferases
RNA Interference
Point Mutation
Histones
Lysine
Catalytic Domain
methyltransferases
point mutation
histones
active sites
binding proteins

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes. / Ding, Yong; Ndamukong, Ivan; Xu, Zaoshi; Lapko, Hanna; Fromm, Michael E; Avramova, Zoya V.

In: PLoS genetics, Vol. 8, No. 12, e1003111, 01.12.2012.

Research output: Contribution to journalArticle

Ding, Yong ; Ndamukong, Ivan ; Xu, Zaoshi ; Lapko, Hanna ; Fromm, Michael E ; Avramova, Zoya V. / ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes. In: PLoS genetics. 2012 ; Vol. 8, No. 12.
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