Attenuation of oxidative stress and alteration of hepatic tissue ultrastructure by D-pinitol in streptozotocin-induced diabetic rats

Selvaraj Sivakumar, Periyasamy Palsamy, Sorimuthu Pillai Subramanian

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The present study was aimed to investigate the effect of D-pinitol on hyperglycaemia mediated oxidative stress by analysing the hepatic antioxidant competence, pro-inflammatory cytokines and ultrastructural changes in liver tissues of streptozotocin-induced diabetic rats. Oral administration of D-pinitol (50 mg/kg b.w.) resulted in significant (p < 0.05) attenuation in blood glucose, glycosylated haemoglobin and pro-inflammatory markers such as TNF-α, IL-1β, IL-6, NF-κB p65 unit and NO and significant (p < 0.05) elevation in the plasma insulin level. In addition, D-pinitol instigated a significant escalation in the levels of hepatic tissue non-enzymatic antioxidants and the activities enzymatic antioxidants of diabetic rats with significant (p < 0.05) decrease in lipid peroxides and hydroperoxides formation, thus demonstrating the protective role of D-pinitol on the hepatic tissues from oxidative stress-induced liver damage. These biochemical observations were complemented by histological and ultrastructural examination of liver section. Thus, the present study demonstrates the hepatoprotective nature of D-pinitol by attenuating hyperglycaemia-mediated pro-inflammatory cytokines and oxidative stress.

Original languageEnglish (US)
Pages (from-to)668-678
Number of pages11
JournalFree Radical Research
Volume44
Issue number6
DOIs
Publication statusPublished - Jun 21 2010

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Keywords

  • D-pinitol
  • Diabetes mellitus
  • Lipid peroxidation
  • Oxidative stress
  • Pro-inflammatory cytokines
  • Streptozotocin

ASJC Scopus subject areas

  • Biochemistry

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