ATP stimulated cyclic AMP formation in bovine chromaffin cells is enhanced by neuropeptide Y

Peijin Zhang, Jialin Zheng, Michael E. Bradley, Terry D. Hexum

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

ATP increases cAMP formation in bovine chromaffin cells, EC50 = 7.1 × 10-6 M. NPY, EC50 = 4.1 × 10-8 M, increases the efficacy of ATP (1.5-2 fold). Inclusion of the selective Y1 receptor antagonist 1229U91 produced a decrease in NPY potency (EC50 = 2.7 × 10-7 M). PTX pretreatment did not abolish either the effect of ATP nor the enhancement by NPY. NPY could also enhance the ability of angiotensin and bradykinin to increase cAMP formation. The selective phospholipase C inhibitor, U73122, and the selective protein kinase C inhibitors, bisindolylmaleimide I and RO-31-8425, were effective inhibitors of the enhancing effect of NPY.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalPeptides
Volume22
Issue number3
DOIs
StatePublished - Apr 11 2001

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Keywords

  • Chromaffin cell
  • Crosstalk
  • G protein
  • Receptor
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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