ATP release by infected bovine monocytes increases the intracellular survival of Mycobacterium avium subsp. paratuberculosis

Seng Ryong Woo, Raul G Barletta, Charles J. Czuprynski

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne's disease, a chronic intestinal infection in ruminants. Adenosine 5′-Triphosphate (ATP) has been reported to induce killing of several Mycobacterium species in human and murine macrophages. We investigated whether ATP secreted from M. avium subsp. paratuberculosis-infected bovine monocytes affects intracellular survival of the bacilli. Bovine monocytes constitutively secreted ATP during an 8-day incubation period in vitro; however, M. avium subsp. paratuberculosis infection did not enhance ATP release. Removal of extracellular ATP by the addition of apyrase increased the viability of infected monocytes, but surprisingly decreased the number of viable intracellular bacilli. In contrast to previous reports, addition of extracellular ATP (1 mM) increased intracellular survival of M. avium subsp. paratuberculosis in bovine monocytes. Neither apyrase nor ATP altered production of reactive oxygen intermediates (ROI) or reactive nitrogen intermediates (RNI) by bovine monocytes. These results suggest that ATP release from infected bovine monocytes improves, rather than decreases, the intracellular survival of M. avium subsp. paratuberculosis.

Original languageEnglish (US)
Pages (from-to)365-377
Number of pages13
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume32
Issue number5
DOIs
StatePublished - Sep 1 2009

Fingerprint

Paratuberculosis
Mycobacterium avium
adenosine triphosphate
Mycobacterium avium subsp. paratuberculosis
monocytes
Monocytes
Adenosine Triphosphate
Survival
cattle
apyrase
Apyrase
Bacillus
paratuberculosis
Ruminants
Mycobacterium
Infection
infection
ruminants
macrophages
Nitrogen

Keywords

  • ATP
  • Bovine
  • Monocytes
  • Mycobacterium subsp. paratuberculosis
  • Purinergic

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

ATP release by infected bovine monocytes increases the intracellular survival of Mycobacterium avium subsp. paratuberculosis. / Woo, Seng Ryong; Barletta, Raul G; Czuprynski, Charles J.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 32, No. 5, 01.09.2009, p. 365-377.

Research output: Contribution to journalArticle

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