Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Yuri L. Lyubchenko, Bertram L. Jacobs, Stuart M. Lindsay

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

Original languageEnglish (US)
Pages (from-to)3983-3986
Number of pages4
JournalNucleic acids research
Volume20
Issue number15
DOIs
StatePublished - Aug 11 1992

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Double-Stranded RNA
Atomic Force Microscopy
Electron Microscopy
Electrophoresis
Buffers
Gels
mica

ASJC Scopus subject areas

  • Genetics

Cite this

Atomic force microscopy of reovirus dsRNA : A routine technique for length measurements. / Lyubchenko, Yuri L.; Jacobs, Bertram L.; Lindsay, Stuart M.

In: Nucleic acids research, Vol. 20, No. 15, 11.08.1992, p. 3983-3986.

Research output: Contribution to journalArticle

Lyubchenko, Yuri L. ; Jacobs, Bertram L. ; Lindsay, Stuart M. / Atomic force microscopy of reovirus dsRNA : A routine technique for length measurements. In: Nucleic acids research. 1992 ; Vol. 20, No. 15. pp. 3983-3986.
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