Atomic force microscopy of nucleoprotein complexes

Y. L. Lyubchenko, B. L. Jacobs, S. M. Lindsay, A. Stasiak, H. G. Hansma, W. Fritzsche, A. Schaper, Z. Shao

Research output: Contribution to journalReview article

84 Citations (Scopus)

Abstract

Recent data on the AFM studies of nucleoprotein complexes of different types are reviewed in this paper. The first section describes the progress in the sample preparation methods for AFM studies of nucleic acids and nucleoprotein complexes. The second part of this paper reviews AFM data on studies of complexes of DNA with regulatory proteins. These studies include two different types of DNA distortion induced by proteins binding: local bending of DNA at sites of protein binding and formation of large loops due to protein-protein interactions between molecules bound to distant sites along the DNA molecules (DNA looping). The prospects for use of AFM for physical mapping of genomes are discussed in this section as well. The third part of the paper reviews data on studies of complexes of DNA with non- sequence specific binding proteins. Special emphasis is given to studies of chromatin which have resulted in progress in the understanding of structure of native chromatin fiber. In this section, novel data on AFM studies of RecA-DNA filaments and complexes of dsRNA with the dsRNA-specific protein p25 are also presented. Discussion of the substrate preparation procedures in relation to the AFM studies of nucleoprotein complexes is given in the final section.

Original languageEnglish (US)
Pages (from-to)705-727
Number of pages23
JournalScanning Microscopy
Volume9
Issue number3
StatePublished - Dec 1 1995

Fingerprint

Atomic force microscopy
DNA
deoxyribonucleic acid
atomic force microscopy
proteins
Proteins
chromatin
preparation
Molecules
genome
Nucleic acids
nucleic acids
molecules
filaments
Genes
fibers
Fibers
Substrates
interactions

Keywords

  • Atomic force microscope
  • DNA
  • RNA
  • RecA-DNA complex
  • mica modification
  • nucleoprotein
  • structure

ASJC Scopus subject areas

  • Instrumentation

Cite this

Lyubchenko, Y. L., Jacobs, B. L., Lindsay, S. M., Stasiak, A., Hansma, H. G., Fritzsche, W., ... Shao, Z. (1995). Atomic force microscopy of nucleoprotein complexes. Scanning Microscopy, 9(3), 705-727.

Atomic force microscopy of nucleoprotein complexes. / Lyubchenko, Y. L.; Jacobs, B. L.; Lindsay, S. M.; Stasiak, A.; Hansma, H. G.; Fritzsche, W.; Schaper, A.; Shao, Z.

In: Scanning Microscopy, Vol. 9, No. 3, 01.12.1995, p. 705-727.

Research output: Contribution to journalReview article

Lyubchenko, YL, Jacobs, BL, Lindsay, SM, Stasiak, A, Hansma, HG, Fritzsche, W, Schaper, A & Shao, Z 1995, 'Atomic force microscopy of nucleoprotein complexes', Scanning Microscopy, vol. 9, no. 3, pp. 705-727.
Lyubchenko YL, Jacobs BL, Lindsay SM, Stasiak A, Hansma HG, Fritzsche W et al. Atomic force microscopy of nucleoprotein complexes. Scanning Microscopy. 1995 Dec 1;9(3):705-727.
Lyubchenko, Y. L. ; Jacobs, B. L. ; Lindsay, S. M. ; Stasiak, A. ; Hansma, H. G. ; Fritzsche, W. ; Schaper, A. ; Shao, Z. / Atomic force microscopy of nucleoprotein complexes. In: Scanning Microscopy. 1995 ; Vol. 9, No. 3. pp. 705-727.
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