Atomic force microscopy characterization of collagen 'nanostraws' in human costal cartilage

M. Stacey, D. Dutta, W. Cao, A. Asmar, H. Elsayed-Ali, R. Kelly, A. Beskok

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Costal cartilage, a type of hyaline cartilage that bridges the bony ribs and sternum, is relatively understudied compared to the load bearing cartilages. Deformities of costal cartilage can result in deformation of the chest wall, where the sternum is largely pushed toward or away from the spine, pectus excavatum and pectus carinatum, respectively, with each condition having significant clinical impact. In the absence of extensive literature describing morphological features of costal cartilage, we characterized a sample from the costal margin immunohistologically and through atomic force microscopy. We had previously observed the presence of collagen 'nanostraws' running the length of costal cartilage. Hypothesizing that these structures may be responsible for fluid flow within this thick, avascular tissue, and prior to microfluidic analysis, we estimated the diameters and measured Young's modulus of elasticity of the collagen nanostraws. We found significant differences in results between treatment type and fixation. Significant differences in nanostraw elasticity and diameter obviously affect nano-fluidic transport calculations, and therefore, we consider these results of importance to the scientific community relying upon measurements in the nanoscale.

Original languageEnglish (US)
Pages (from-to)483-487
Number of pages5
JournalMicron
Volume44
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

cartilage
Atomic Force Microscopy
Cartilage
collagens
Collagen
Atomic force microscopy
atomic force microscopy
Sternum
Elastic Modulus
sternum
Funnel Chest
Hyaline Cartilage
Microfluidic Analytical Techniques
modulus of elasticity
Elasticity
Weight-Bearing
Thoracic Wall
Ribs
Bearings (structural)
Elastic moduli

Keywords

  • AFM
  • Cartilage
  • Collagen fibers

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

Cite this

Atomic force microscopy characterization of collagen 'nanostraws' in human costal cartilage. / Stacey, M.; Dutta, D.; Cao, W.; Asmar, A.; Elsayed-Ali, H.; Kelly, R.; Beskok, A.

In: Micron, Vol. 44, No. 1, 01.01.2013, p. 483-487.

Research output: Contribution to journalArticle

Stacey, M, Dutta, D, Cao, W, Asmar, A, Elsayed-Ali, H, Kelly, R & Beskok, A 2013, 'Atomic force microscopy characterization of collagen 'nanostraws' in human costal cartilage', Micron, vol. 44, no. 1, pp. 483-487. https://doi.org/10.1016/j.micron.2012.10.006
Stacey, M. ; Dutta, D. ; Cao, W. ; Asmar, A. ; Elsayed-Ali, H. ; Kelly, R. ; Beskok, A. / Atomic force microscopy characterization of collagen 'nanostraws' in human costal cartilage. In: Micron. 2013 ; Vol. 44, No. 1. pp. 483-487.
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