ATA2-mediated amino acid uptake following partial hepatectomy is regulated by redistribution to the plasma membrane

T. L. Freeman, Geoffrey Milton Thiele, D. J. Tuma, T. K. Machu, Mark E Mailliard

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

System A, the Na+-dependent amino acid transport activity, is encoded by the ATA2 gene and up-regulated following partial hepatectomy (PH), and its competitive inhibition interferes with liver regeneration. Rabbit polyclonal antibody was raised against a portion of the ATA2 gene product followed by immunodetection of ATA2 in isolated liver plasma membrane and lysate. The level of ATA2 increased in the plasma membrane following PH, while the relatively high quantity of ATA2 found in liver lysate remained constant. We also have shown that Northern analysis of steady-state ATA2 mRNA revealed no significant change following PH. These data show that ATA2-mediated transport is not regulated by the steady-state level of ATA2 mRNA but is regulated by the amount of ATA2 and redistribution to the plasma membrane. We hypothesize that ATA2 activity is regulated by recruitment of ATA2 protein from an intracellular compartment. In addition, the pattern of expression of System A activity in oocytes, transport kinetics, and sensitivity to chemical modification indicate the presence of a second System A isoform in liver that differs substantially from ATA2.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume400
Issue number2
DOIs
StatePublished - Apr 15 2002

Fingerprint

Hepatectomy
Cell membranes
Liver
Cell Membrane
Amino Acids
Messenger RNA
Liver Regeneration
Genes
Oocytes
Chemical modification
Protein Isoforms
Rabbits
Antibodies
Kinetics
Proteins

Keywords

  • ATA2
  • Amino acid transport
  • Liver regeneration
  • Plasma membrane
  • System A

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

ATA2-mediated amino acid uptake following partial hepatectomy is regulated by redistribution to the plasma membrane. / Freeman, T. L.; Thiele, Geoffrey Milton; Tuma, D. J.; Machu, T. K.; Mailliard, Mark E.

In: Archives of Biochemistry and Biophysics, Vol. 400, No. 2, 15.04.2002, p. 215-222.

Research output: Contribution to journalArticle

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