Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells

Xiaoxia Wang, Jennifer L. Pluznick, Deann C. Settles, Steven Claude Sansom

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We tested the hypotheses that the NO-cGMP-PKG pathway mediates inhibition of the store-operated cation channel (SOC) in human glomerular mesangial cells (HMC) and that TRPC4, a molecular component of SOC in HMC, is associated with PKG-phosphorylated vasodilator-stimulated phosphoprotein (VASP). Using fura 2 ratiometry, we measured intracellular Ca2+ concentration [Ca 2+]i to determine whether sodium nitroprusside (SNP), an NO donor, and 8-Br-cGMP affected SOC-TRPC4 via PKG. We found that the SOC response in HMC was attenuated in the presence of 100 μM SNP, an NO donor, or 100 μM 8-Br-cGMP. Addition of DT-3 (2.5 μM), a specific PKG-1α inhibitor, reversed the effects of 8-Br-cGMP on the SOC response. Application of 100 μM cAMP did not significantly inhibit the SOC response. RT-PCR and Western blotting revealed PKG-1α transcript and protein in HMC. Immunocytochemical analysis localized PKG-1α to the cytoplasm and plasma membrane of HMC. Previous studies have shown that PKG-mediated phosphorylation of VASP attenuates cellular Ca2+ entry, resulting in altered growth and proliferation. Therefore, we used Western blotting and immunocytochemistry to determine whether PKG-phosphorylated VASP associates with TRPC4. Western blot analysis revealed that 8-Br-cGMP enhanced the phosphorylation of VASP at serine 239 (Ser239), a known PKG phosphorylation site, in HMC within 5 min. Coimmunoprecipitation and coimmunostaining showed that P-Ser239-VASP associated with TRPC4. However, VASP that was unphosphorylated at Ser239 was not associated with TRPC4. These results indicate that VASP has a role in the NO/PKG-1α-mediated inhibition of the TRPC4-SOC response in HMC.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number6
DOIs
StatePublished - Dec 1 2007

Fingerprint

Mesangial Cells
Cations
Calcium
Serine
Western Blotting
Phosphorylation
Nitroprusside
Fura-2
vasodilator-stimulated phosphoprotein
Cytoplasm
Immunohistochemistry
Cell Membrane
Polymerase Chain Reaction
Growth

Keywords

  • Nitric oxide
  • SNP
  • SOC
  • cGMP

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells. / Wang, Xiaoxia; Pluznick, Jennifer L.; Settles, Deann C.; Sansom, Steven Claude.

In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 6, 01.12.2007.

Research output: Contribution to journalArticle

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abstract = "We tested the hypotheses that the NO-cGMP-PKG pathway mediates inhibition of the store-operated cation channel (SOC) in human glomerular mesangial cells (HMC) and that TRPC4, a molecular component of SOC in HMC, is associated with PKG-phosphorylated vasodilator-stimulated phosphoprotein (VASP). Using fura 2 ratiometry, we measured intracellular Ca2+ concentration [Ca 2+]i to determine whether sodium nitroprusside (SNP), an NO donor, and 8-Br-cGMP affected SOC-TRPC4 via PKG. We found that the SOC response in HMC was attenuated in the presence of 100 μM SNP, an NO donor, or 100 μM 8-Br-cGMP. Addition of DT-3 (2.5 μM), a specific PKG-1α inhibitor, reversed the effects of 8-Br-cGMP on the SOC response. Application of 100 μM cAMP did not significantly inhibit the SOC response. RT-PCR and Western blotting revealed PKG-1α transcript and protein in HMC. Immunocytochemical analysis localized PKG-1α to the cytoplasm and plasma membrane of HMC. Previous studies have shown that PKG-mediated phosphorylation of VASP attenuates cellular Ca2+ entry, resulting in altered growth and proliferation. Therefore, we used Western blotting and immunocytochemistry to determine whether PKG-phosphorylated VASP associates with TRPC4. Western blot analysis revealed that 8-Br-cGMP enhanced the phosphorylation of VASP at serine 239 (Ser239), a known PKG phosphorylation site, in HMC within 5 min. Coimmunoprecipitation and coimmunostaining showed that P-Ser239-VASP associated with TRPC4. However, VASP that was unphosphorylated at Ser239 was not associated with TRPC4. These results indicate that VASP has a role in the NO/PKG-1α-mediated inhibition of the TRPC4-SOC response in HMC.",
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AB - We tested the hypotheses that the NO-cGMP-PKG pathway mediates inhibition of the store-operated cation channel (SOC) in human glomerular mesangial cells (HMC) and that TRPC4, a molecular component of SOC in HMC, is associated with PKG-phosphorylated vasodilator-stimulated phosphoprotein (VASP). Using fura 2 ratiometry, we measured intracellular Ca2+ concentration [Ca 2+]i to determine whether sodium nitroprusside (SNP), an NO donor, and 8-Br-cGMP affected SOC-TRPC4 via PKG. We found that the SOC response in HMC was attenuated in the presence of 100 μM SNP, an NO donor, or 100 μM 8-Br-cGMP. Addition of DT-3 (2.5 μM), a specific PKG-1α inhibitor, reversed the effects of 8-Br-cGMP on the SOC response. Application of 100 μM cAMP did not significantly inhibit the SOC response. RT-PCR and Western blotting revealed PKG-1α transcript and protein in HMC. Immunocytochemical analysis localized PKG-1α to the cytoplasm and plasma membrane of HMC. Previous studies have shown that PKG-mediated phosphorylation of VASP attenuates cellular Ca2+ entry, resulting in altered growth and proliferation. Therefore, we used Western blotting and immunocytochemistry to determine whether PKG-phosphorylated VASP associates with TRPC4. Western blot analysis revealed that 8-Br-cGMP enhanced the phosphorylation of VASP at serine 239 (Ser239), a known PKG phosphorylation site, in HMC within 5 min. Coimmunoprecipitation and coimmunostaining showed that P-Ser239-VASP associated with TRPC4. However, VASP that was unphosphorylated at Ser239 was not associated with TRPC4. These results indicate that VASP has a role in the NO/PKG-1α-mediated inhibition of the TRPC4-SOC response in HMC.

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