Association of group B Streptococcus colonization and bovine exposure: A prospective cross-sectional cohort study

Shannon D. Manning, A. Cody Springman, Amber D. Million, Nicole R. Milton, Sara E. McNamara, Patricia A. Somsel, Paul Bartlett, H. Dele Davies

Research output: Contribution to journalArticle

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Abstract

Background: While Group B Streptococcus (GBS) human colonization and infection has long been suspected as originating from cows, several investigators have suggested that ongoing interspecies GBS transmission is unlikely due to genotyping data demonstrating that human and bovine-derived GBS strains represent mostly distinct populations. The possibility of ongoing transmission between humans and their livestock has not been systematically examined. Methodology/Principal Findings: To examine ongoing interspecies transmission, we conducted a prospective crosssectional cohort study of 68 families and their livestock. Stool specimens were collected from 154 people and 115 livestock; GBS was detected in 19 (12.3%) humans and 2 (1.7%) animals (bovine and sheep). Application of multilocus sequence typing (MLST) identified 8 sequence types (STs or clones), with STs 1 and 23 predominating. There were 11 families in which two members submitted stools and at least one had GBS colonization. In 3 of these families, both members (consisting of couples) were colonized, yielding a co-colonization rate of 27% (95% CI: 7%-61%). Two of these couples had strains with identical MLST, capsule (cps) genotype, susceptibility, and RAPD profiles. One couple co-colonized with ST-1 (cps5) strains also had a bovine colonized with the identical strain type. On multivariate analysis of questionnaire data, cattle exposure was a predictor of GBS colonization, with each unit increase in days of cattle exposure increasing the odds of colonization by 20% (P = 0.02). These results support interspecies transmission with additional evidence for transmission provided by the epidemiological association with cattle exposure. Conclusions/Significance: Although GBS uncommonly colonizes livestock stools, increased frequency of cattle exposure was significantly associated with human colonization and one couple shared the same GBS strains as their bovine suggesting intraspecies transmission. These results set the framework for GBS as a possible zoonotic infection, which has significant public health implications.

Original languageEnglish (US)
Article numbere8795
JournalPloS one
Volume5
Issue number1
DOIs
StatePublished - Jan 20 2010

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Streptococcus agalactiae
cohort studies
Cohort Studies
Cross-Sectional Studies
Agriculture
cattle
Livestock
livestock
Multilocus Sequence Typing
Public health
Capsules
Animals
Zoonoses
infection
genotyping
multivariate analysis
Sheep
public health
Multivariate Analysis
questionnaires

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Manning, S. D., Springman, A. C., Million, A. D., Milton, N. R., McNamara, S. E., Somsel, P. A., ... Davies, H. D. (2010). Association of group B Streptococcus colonization and bovine exposure: A prospective cross-sectional cohort study. PloS one, 5(1), [e8795]. https://doi.org/10.1371/journal.pone.0008795

Association of group B Streptococcus colonization and bovine exposure : A prospective cross-sectional cohort study. / Manning, Shannon D.; Springman, A. Cody; Million, Amber D.; Milton, Nicole R.; McNamara, Sara E.; Somsel, Patricia A.; Bartlett, Paul; Davies, H. Dele.

In: PloS one, Vol. 5, No. 1, e8795, 20.01.2010.

Research output: Contribution to journalArticle

Manning, SD, Springman, AC, Million, AD, Milton, NR, McNamara, SE, Somsel, PA, Bartlett, P & Davies, HD 2010, 'Association of group B Streptococcus colonization and bovine exposure: A prospective cross-sectional cohort study', PloS one, vol. 5, no. 1, e8795. https://doi.org/10.1371/journal.pone.0008795
Manning, Shannon D. ; Springman, A. Cody ; Million, Amber D. ; Milton, Nicole R. ; McNamara, Sara E. ; Somsel, Patricia A. ; Bartlett, Paul ; Davies, H. Dele. / Association of group B Streptococcus colonization and bovine exposure : A prospective cross-sectional cohort study. In: PloS one. 2010 ; Vol. 5, No. 1.
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