Association of a DNA marker with Hessian fly resistance gene H9 in wheat

I. Dweikat, H. Ohm, S. Mackenzie, F. Patterson, S. Cambron, R. Ratcliffe

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.

Original languageEnglish (US)
Pages (from-to)964-968
Number of pages5
JournalTheoretical and Applied Genetics
Volume89
Issue number7-8
DOIs
StatePublished - Dec 1 1994

Fingerprint

Mayetiola destructor
Genetic Markers
Diptera
Triticum
wheat
genetic markers
random amplified polymorphic DNA technique
Genes
denaturing gradient gel electrophoresis
Denaturing Gradient Gel Electrophoresis
biotypes
genes
Triticum aestivum
genetic resistance
durum wheat
DNA
genetic background
hybridization
gels
pests

Keywords

  • DNA markers
  • Hessian fly
  • RAPD
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Dweikat, I., Ohm, H., Mackenzie, S., Patterson, F., Cambron, S., & Ratcliffe, R. (1994). Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Theoretical and Applied Genetics, 89(7-8), 964-968. https://doi.org/10.1007/BF00224525

Association of a DNA marker with Hessian fly resistance gene H9 in wheat. / Dweikat, I.; Ohm, H.; Mackenzie, S.; Patterson, F.; Cambron, S.; Ratcliffe, R.

In: Theoretical and Applied Genetics, Vol. 89, No. 7-8, 01.12.1994, p. 964-968.

Research output: Contribution to journalArticle

Dweikat, I, Ohm, H, Mackenzie, S, Patterson, F, Cambron, S & Ratcliffe, R 1994, 'Association of a DNA marker with Hessian fly resistance gene H9 in wheat', Theoretical and Applied Genetics, vol. 89, no. 7-8, pp. 964-968. https://doi.org/10.1007/BF00224525
Dweikat I, Ohm H, Mackenzie S, Patterson F, Cambron S, Ratcliffe R. Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Theoretical and Applied Genetics. 1994 Dec 1;89(7-8):964-968. https://doi.org/10.1007/BF00224525
Dweikat, I. ; Ohm, H. ; Mackenzie, S. ; Patterson, F. ; Cambron, S. ; Ratcliffe, R. / Association of a DNA marker with Hessian fly resistance gene H9 in wheat. In: Theoretical and Applied Genetics. 1994 ; Vol. 89, No. 7-8. pp. 964-968.
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