Assessment of genotoxicity of nicotine employing in vitro mammalian test system

A. H. Trivedi, Bhavana J Dave, S. G. Adhvaryu

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Genotoxicity of nicotine was evaluated employing Chinese hamster ovary (CHO) cells. Two cytogenetic endpoints, viz. frequency of sister chromatid exchange (SCE) and chromosome aberration (CA) were considered. Nicotine was found to induce CA and SCE frequency in a dose and duration dependent manner. Statistically significant elevations in CA frequency were observed only with higher concentrations of nicotine, whereas, SCE frequencies were increased significantly by all the doses utilized. It was genotoxic at the concentration, comparable to the saliva levels of nicotine achieved during tobacco chewing. The results obtained by continuous and pulse treatments with nicotine explain the harmful effects of chronic tobacco consumption.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalCancer Letters
Volume54
Issue number1-2
DOIs
StatePublished - Oct 8 1990

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Nicotine
Sister Chromatid Exchange
Chromosome Aberrations
Tobacco Use
Cricetulus
Saliva
Cytogenetics
Ovary
In Vitro Techniques

Keywords

  • Chinese hamster ovary cells
  • chromosome aberration
  • genotoxicity
  • nicotine
  • sister chromatid exchange

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Assessment of genotoxicity of nicotine employing in vitro mammalian test system. / Trivedi, A. H.; Dave, Bhavana J; Adhvaryu, S. G.

In: Cancer Letters, Vol. 54, No. 1-2, 08.10.1990, p. 89-94.

Research output: Contribution to journalArticle

Trivedi, A. H. ; Dave, Bhavana J ; Adhvaryu, S. G. / Assessment of genotoxicity of nicotine employing in vitro mammalian test system. In: Cancer Letters. 1990 ; Vol. 54, No. 1-2. pp. 89-94.
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