Assessing carotenoid content in plant leaves with reflectance spectroscopy

Anatoly A. Gitelson, Yoav Zur, Olga B. Chivkunova, Mark N. Merzlyak

Research output: Contribution to journalArticle

325 Citations (Scopus)

Abstract

Spectral reflectance of maple, chestnut and beech leaves in a wide range of pigment content and composition was investigated to devise a nondestructive technique for total carotenoid (Car) content estimation in higher plant leaves. Reciprocal reflectance in the range 510 to 550 nm was found to be closely related to the total pigment content in leaves. The sensitivity of reciprocal reflectance to Car content was maximal in a spectral range around 510 nm; however, chlorophylls (Chl) also affect reflectance in this spectral range. To remove the Chl effect on the reciprocal reflectance at 510 nm, a reciprocal reflectance at either 550 or 700 nm was used, which was linearly proportional to the Chl content. Indices for nondestructive estimation of Car content in leaves were devised and validated. Reflectances in three spectral bands, 510 ± 5 nm, either 550 ± 15 nm or 700 ± 7.5 nm and the near infrared range above 750 nm are sufficient to estimate total Car content in plant leaves nondestructively with a root mean square error of less than 1.75 nmol/cm2.

Original languageEnglish (US)
Pages (from-to)272-281
Number of pages10
JournalPhotochemistry and Photobiology
Volume75
Issue number3
DOIs
StatePublished - Mar 1 2002

Fingerprint

carotenoids
Plant Leaves
Carotenoids
leaves
Spectrum Analysis
Chlorophyll
Spectroscopy
reflectance
spectroscopy
chlorophylls
Acer
Fagus
pigments
Pigments
spectral reflectance
root-mean-square errors
spectral bands
Mean square error
Infrared radiation
sensitivity

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

Assessing carotenoid content in plant leaves with reflectance spectroscopy. / Gitelson, Anatoly A.; Zur, Yoav; Chivkunova, Olga B.; Merzlyak, Mark N.

In: Photochemistry and Photobiology, Vol. 75, No. 3, 01.03.2002, p. 272-281.

Research output: Contribution to journalArticle

Gitelson, Anatoly A. ; Zur, Yoav ; Chivkunova, Olga B. ; Merzlyak, Mark N. / Assessing carotenoid content in plant leaves with reflectance spectroscopy. In: Photochemistry and Photobiology. 2002 ; Vol. 75, No. 3. pp. 272-281.
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