Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice

Allison J. Richard, Thomas P. Burris, David Sanchez-Infantes, Yongjun Wang, David M. Ribnicky, Jacqueline M. Stephens

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Studies have shown that the inability of adipose tissue to properly expand during the obese state or respond to insulin can lead to metabolic dysfunction. Artemisia is a diverse group of plants that has a history of medicinal use. The aim of this study was to examine the ability of ethanolic extracts of Artemisia scoparia (SCO) and Artemisia santolinifolia (SAN) to modulate adipocyte development in cultured adipocytes and white adipose tissue (WAT) function invivo using a mouse model of diet-induced obesity. Method: Adipogenesis was assessed using Oil Red O staining and immunoblotting. A nuclear receptor specificity assay was used to examine the specificity of SCO- and SAN-induced PPARγ activation. C57BL/6J mice, fed a high-fat diet, were gavaged with saline, SCO, or SAN for 2 wk. Whole-body insulin sensitivity was examined using insulin tolerance tests. WAT depots were assessed via immunoblotting for markers of insulin action and adipokine production. Results: We established that SCO and SAN were highly specific activators of PPARγ and did not activate other nuclear receptors. After a 1-wk daily gavage, SCO- and SAN-treated mice had lower insulin-induced glucose disposal rates than control mice. At the end of the 2-wk treatment period, SCO- and SAN-treated mice had enhanced insulin-responsive Akt serine-473 phosphorylation and significantly decreased monocyte chemotactic protein-1 levels in visceral WAT compared with control mice; these differences were depot specific. Moreover, plasma adiponectin levels were increased following SCO treatment. Conclusion: Overall, these studies demonstrate that extracts from two Artemisia species can have metabolically favorable effects on adipocytes and WAT.

Original languageEnglish (US)
Pages (from-to)S31-S36
JournalNutrition
Volume30
Issue number7-8 SUPPL.
DOIs
StatePublished - Jul 2014

Fingerprint

Scoparia
Artemisia
Obese Mice
Adipogenesis
Peroxisome Proliferator-Activated Receptors
Insulin Resistance
Adipose Tissue
White Adipose Tissue
Insulin
Adipocytes
Cytoplasmic and Nuclear Receptors
Immunoblotting
Adipokines
Intra-Abdominal Fat
Chemokine CCL2
Adiponectin
High Fat Diet
Inbred C57BL Mouse
Serine
Obesity

Keywords

  • 3T3-L1 adipocytes
  • Botanicals
  • Diet-induced obesity
  • Fat cells
  • Insulin action

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Richard, A. J., Burris, T. P., Sanchez-Infantes, D., Wang, Y., Ribnicky, D. M., & Stephens, J. M. (2014). Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice. Nutrition, 30(7-8 SUPPL.), S31-S36. https://doi.org/10.1016/j.nut.2014.02.013

Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice. / Richard, Allison J.; Burris, Thomas P.; Sanchez-Infantes, David; Wang, Yongjun; Ribnicky, David M.; Stephens, Jacqueline M.

In: Nutrition, Vol. 30, No. 7-8 SUPPL., 07.2014, p. S31-S36.

Research output: Contribution to journalArticle

Richard, AJ, Burris, TP, Sanchez-Infantes, D, Wang, Y, Ribnicky, DM & Stephens, JM 2014, 'Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice', Nutrition, vol. 30, no. 7-8 SUPPL., pp. S31-S36. https://doi.org/10.1016/j.nut.2014.02.013
Richard, Allison J. ; Burris, Thomas P. ; Sanchez-Infantes, David ; Wang, Yongjun ; Ribnicky, David M. ; Stephens, Jacqueline M. / Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice. In: Nutrition. 2014 ; Vol. 30, No. 7-8 SUPPL. pp. S31-S36.
@article{5d2925d50ec34a9ab18537c46f0cd375,
title = "Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice",
abstract = "Objective: Studies have shown that the inability of adipose tissue to properly expand during the obese state or respond to insulin can lead to metabolic dysfunction. Artemisia is a diverse group of plants that has a history of medicinal use. The aim of this study was to examine the ability of ethanolic extracts of Artemisia scoparia (SCO) and Artemisia santolinifolia (SAN) to modulate adipocyte development in cultured adipocytes and white adipose tissue (WAT) function invivo using a mouse model of diet-induced obesity. Method: Adipogenesis was assessed using Oil Red O staining and immunoblotting. A nuclear receptor specificity assay was used to examine the specificity of SCO- and SAN-induced PPARγ activation. C57BL/6J mice, fed a high-fat diet, were gavaged with saline, SCO, or SAN for 2 wk. Whole-body insulin sensitivity was examined using insulin tolerance tests. WAT depots were assessed via immunoblotting for markers of insulin action and adipokine production. Results: We established that SCO and SAN were highly specific activators of PPARγ and did not activate other nuclear receptors. After a 1-wk daily gavage, SCO- and SAN-treated mice had lower insulin-induced glucose disposal rates than control mice. At the end of the 2-wk treatment period, SCO- and SAN-treated mice had enhanced insulin-responsive Akt serine-473 phosphorylation and significantly decreased monocyte chemotactic protein-1 levels in visceral WAT compared with control mice; these differences were depot specific. Moreover, plasma adiponectin levels were increased following SCO treatment. Conclusion: Overall, these studies demonstrate that extracts from two Artemisia species can have metabolically favorable effects on adipocytes and WAT.",
keywords = "3T3-L1 adipocytes, Botanicals, Diet-induced obesity, Fat cells, Insulin action",
author = "Richard, {Allison J.} and Burris, {Thomas P.} and David Sanchez-Infantes and Yongjun Wang and Ribnicky, {David M.} and Stephens, {Jacqueline M.}",
year = "2014",
month = "7",
doi = "10.1016/j.nut.2014.02.013",
language = "English (US)",
volume = "30",
pages = "S31--S36",
journal = "Nutrition",
issn = "0899-9007",
publisher = "Elsevier Inc.",
number = "7-8 SUPPL.",

}

TY - JOUR

T1 - Artemisia extracts activate PPARγ, promote adipogenesis, and enhance insulin sensitivity in adipose tissue of obese mice

AU - Richard, Allison J.

AU - Burris, Thomas P.

AU - Sanchez-Infantes, David

AU - Wang, Yongjun

AU - Ribnicky, David M.

AU - Stephens, Jacqueline M.

PY - 2014/7

Y1 - 2014/7

N2 - Objective: Studies have shown that the inability of adipose tissue to properly expand during the obese state or respond to insulin can lead to metabolic dysfunction. Artemisia is a diverse group of plants that has a history of medicinal use. The aim of this study was to examine the ability of ethanolic extracts of Artemisia scoparia (SCO) and Artemisia santolinifolia (SAN) to modulate adipocyte development in cultured adipocytes and white adipose tissue (WAT) function invivo using a mouse model of diet-induced obesity. Method: Adipogenesis was assessed using Oil Red O staining and immunoblotting. A nuclear receptor specificity assay was used to examine the specificity of SCO- and SAN-induced PPARγ activation. C57BL/6J mice, fed a high-fat diet, were gavaged with saline, SCO, or SAN for 2 wk. Whole-body insulin sensitivity was examined using insulin tolerance tests. WAT depots were assessed via immunoblotting for markers of insulin action and adipokine production. Results: We established that SCO and SAN were highly specific activators of PPARγ and did not activate other nuclear receptors. After a 1-wk daily gavage, SCO- and SAN-treated mice had lower insulin-induced glucose disposal rates than control mice. At the end of the 2-wk treatment period, SCO- and SAN-treated mice had enhanced insulin-responsive Akt serine-473 phosphorylation and significantly decreased monocyte chemotactic protein-1 levels in visceral WAT compared with control mice; these differences were depot specific. Moreover, plasma adiponectin levels were increased following SCO treatment. Conclusion: Overall, these studies demonstrate that extracts from two Artemisia species can have metabolically favorable effects on adipocytes and WAT.

AB - Objective: Studies have shown that the inability of adipose tissue to properly expand during the obese state or respond to insulin can lead to metabolic dysfunction. Artemisia is a diverse group of plants that has a history of medicinal use. The aim of this study was to examine the ability of ethanolic extracts of Artemisia scoparia (SCO) and Artemisia santolinifolia (SAN) to modulate adipocyte development in cultured adipocytes and white adipose tissue (WAT) function invivo using a mouse model of diet-induced obesity. Method: Adipogenesis was assessed using Oil Red O staining and immunoblotting. A nuclear receptor specificity assay was used to examine the specificity of SCO- and SAN-induced PPARγ activation. C57BL/6J mice, fed a high-fat diet, were gavaged with saline, SCO, or SAN for 2 wk. Whole-body insulin sensitivity was examined using insulin tolerance tests. WAT depots were assessed via immunoblotting for markers of insulin action and adipokine production. Results: We established that SCO and SAN were highly specific activators of PPARγ and did not activate other nuclear receptors. After a 1-wk daily gavage, SCO- and SAN-treated mice had lower insulin-induced glucose disposal rates than control mice. At the end of the 2-wk treatment period, SCO- and SAN-treated mice had enhanced insulin-responsive Akt serine-473 phosphorylation and significantly decreased monocyte chemotactic protein-1 levels in visceral WAT compared with control mice; these differences were depot specific. Moreover, plasma adiponectin levels were increased following SCO treatment. Conclusion: Overall, these studies demonstrate that extracts from two Artemisia species can have metabolically favorable effects on adipocytes and WAT.

KW - 3T3-L1 adipocytes

KW - Botanicals

KW - Diet-induced obesity

KW - Fat cells

KW - Insulin action

UR - http://www.scopus.com/inward/record.url?scp=84905900988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905900988&partnerID=8YFLogxK

U2 - 10.1016/j.nut.2014.02.013

DO - 10.1016/j.nut.2014.02.013

M3 - Article

C2 - 24985103

AN - SCOPUS:84905900988

VL - 30

SP - S31-S36

JO - Nutrition

JF - Nutrition

SN - 0899-9007

IS - 7-8 SUPPL.

ER -