Arginine vasotocin gene expression and secretion during osmotic stimulation and hemorrhagic hypotension in hens

Shmuel Jaccoby, Amar Bahadur Singh, Lawrence E. Cornett, Thomas I. Koike

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In chickens, hyperosmolality stimulates the secretion of vasotocin (AVT) and up-regulates hypothalamic AVT gene expression. Hemorrhage, on the other hand, has not been considered an effective stimulus for AVT release in this species. The effects of acute osmotic stress and prolonged hemorrhagic hypotension on AVT gene expression and secretion were studied in White Leghorn hens. Conscious hens were osmotically stimulated by administering a single ip injection of 3 M NaCl (5 ml/kg). Urethane-anesthetized hens were bled to a mean arterial pressure of 80-90 mm Hg and the pressure was maintained within this range by additional bleeding. A total of about 30% of the estimated blood volume was removed. Both experiments were terminated after 1 hr of stimulation. Plasma AVT levels in the hyperosmotic and hypovolemic hens were 4- and 2-fold higher, respectively, compared to controls. Hypothalamic AVT mRNA levels, detected by Northern blot analysis, were 2.5- and 2-fold higher in the osmotically stimulated and hypotensive groups, respectively, compared to control groups. As determined by in situ hybridization, both osmotic stimulation and hypovolemia resulted in an increase in the number of AVT mRNA-containing neurons in the supraoptic and paraventricular nuclei. Our results indicate that, under the conditions used, hypotension and hyperosmolality are equally effective in stimulating AVT gene expression and secretion of AVT.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalGeneral and Comparative Endocrinology
Volume106
Issue number3
DOIs
StatePublished - Jan 1 1997

Fingerprint

arginine vasotocin
Vasotocin
hypotension
Hypotension
hens
Hypovolemia
secretion
Gene Expression
gene expression
Hemorrhage
hemorrhage
Supraoptic Nucleus
Messenger RNA
Paraventricular Hypothalamic Nucleus
vasotocin
Urethane
Osmotic Pressure
hypovolemic shock
Blood Volume
urethane

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Cite this

Arginine vasotocin gene expression and secretion during osmotic stimulation and hemorrhagic hypotension in hens. / Jaccoby, Shmuel; Singh, Amar Bahadur; Cornett, Lawrence E.; Koike, Thomas I.

In: General and Comparative Endocrinology, Vol. 106, No. 3, 01.01.1997, p. 327-337.

Research output: Contribution to journalArticle

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