Arabidopsis Trithorax1 dynamically regulates Flowering Locus C activation via histone 3 lysine 4 trimethylation

Stéphane Pien, Delphine Fleury, Joshua S. Mylne, Pedro Crevillen, Dirk Inzé, Zoya V Avramova, Caroline Dean, Ueli Grossniklaus

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Trithorax function is essential for epigenetic maintenance of gene expression in animals, but little is known about trithorax homologs in plants. ARABIDOPSIS TRITHORAX1 (ATX1) was shown to be required for the expression of homeotic genes involved in flower organogenesis. Here, we report a novel function of ATX1, namely, the epigenetic regulation of the floral repressor FLOWERING LOCUS C (FLC). Downregulation of FLC accelerates the transition from vegetative to reproductive development in Arabidopsis thaliana. In the atx1 mutant, FLC levels are reduced and the FLC chromatin is depleted of trimethylated, but not dimethylated, histone 3 lysine 4, suggesting a specific trimethylation function of ATX1. In addition, we found that ATX1 directly binds the active FLC locus before flowering and that this interaction is released upon the transition to flowering. This dynamic process stands in contrast with the stable maintenance of homeotic gene expression mediated by trithorax group proteins in animals but resembles the dynamics of plant Polycomb group function.

Original languageEnglish (US)
Pages (from-to)580-588
Number of pages9
JournalPlant Cell
Volume20
Issue number3
DOIs
StatePublished - Mar 1 2008

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Arabidopsis
histones
Histones
Lysine
lysine
Chemical activation
flowering
loci
homeotic genes
Gene expression
epigenetics
Homeobox Genes
Animals
Epigenomics
gene expression
Maintenance
animal proteins
Gene Expression
organogenesis
Organogenesis

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Arabidopsis Trithorax1 dynamically regulates Flowering Locus C activation via histone 3 lysine 4 trimethylation. / Pien, Stéphane; Fleury, Delphine; Mylne, Joshua S.; Crevillen, Pedro; Inzé, Dirk; Avramova, Zoya V; Dean, Caroline; Grossniklaus, Ueli.

In: Plant Cell, Vol. 20, No. 3, 01.03.2008, p. 580-588.

Research output: Contribution to journalArticle

Pien, S, Fleury, D, Mylne, JS, Crevillen, P, Inzé, D, Avramova, ZV, Dean, C & Grossniklaus, U 2008, 'Arabidopsis Trithorax1 dynamically regulates Flowering Locus C activation via histone 3 lysine 4 trimethylation', Plant Cell, vol. 20, no. 3, pp. 580-588. https://doi.org/10.1105/tpc.108.058172
Pien, Stéphane ; Fleury, Delphine ; Mylne, Joshua S. ; Crevillen, Pedro ; Inzé, Dirk ; Avramova, Zoya V ; Dean, Caroline ; Grossniklaus, Ueli. / Arabidopsis Trithorax1 dynamically regulates Flowering Locus C activation via histone 3 lysine 4 trimethylation. In: Plant Cell. 2008 ; Vol. 20, No. 3. pp. 580-588.
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