Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis

Corey S. Westfall, Ashley M. Sherp, Chloe Zubieta, Sophie Alvarez, Evelyn Schraft, Romain Marcellin, Loren Ramirez, Joseph M. Jez

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In Arabidopsis thaliana, the acyl acid amido synthetase Gretchen Hagen 3.5 (AtGH3.5) conjugates both indole-3-acetic acid (IAA) and salicylic acid (SA) to modulate auxin and pathogen response pathways. To understand the molecular basis for the activity of AtGH3.5, we determined the X-ray crystal structure of the enzyme in complex with IAA and AMP. Biochemical analysis demonstrates that the substrate preference of AtGH3.5 is wider than originally described and includes the natural auxin phenylacetic acid (PAA) and the potential SA precursor benzoic acid (BA). Residues that determine IAA versus BA substrate preference were identified. The dual functionality of AtGH3.5 is unique to this enzyme although multiple IAA-conjugating GH3 proteins share nearly identical acyl acid binding sites. In planta analysis of IAA, PAA, SA, and BA and their respective aspartyl conjugates were determined in wild-type and overexpressing lines of A. thaliana. This study suggests that AtGH3.5 conjugates auxins (i.e., IAA and PAA) and benzoates (i.e., SA and BA) to mediate crosstalk between different metabolic pathways, broadening the potential roles for GH3 acyl acid amido synthetases in plants.

Original languageEnglish (US)
Pages (from-to)13917-13922
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number48
DOIs
StatePublished - Nov 29 2016

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Indoleacetic Acids
Salicylic Acid
Ligases
Arabidopsis
Homeostasis
Benzoic Acid
Acids
Benzoates
Enzymes
Adenosine Monophosphate
Metabolic Networks and Pathways
indoleacetic acid
Binding Sites
X-Rays
phenylacetic acid
Proteins

Keywords

  • Arabidopsis
  • Auxin
  • Plant biochemistry
  • Plant hormone
  • Protein structure

ASJC Scopus subject areas

  • General

Cite this

Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis. / Westfall, Corey S.; Sherp, Ashley M.; Zubieta, Chloe; Alvarez, Sophie; Schraft, Evelyn; Marcellin, Romain; Ramirez, Loren; Jez, Joseph M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 48, 29.11.2016, p. 13917-13922.

Research output: Contribution to journalArticle

Westfall, Corey S. ; Sherp, Ashley M. ; Zubieta, Chloe ; Alvarez, Sophie ; Schraft, Evelyn ; Marcellin, Romain ; Ramirez, Loren ; Jez, Joseph M. / Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 48. pp. 13917-13922.
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