Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during Turnip mosaic virus infection

Hernan Garcia-Ruiz, Atsushi Takeda, Elisabeth J. Chapman, Christopher M. Sullivan, Noah Fahlgren, Katherine J. Brempelis, James C. Carrington

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Plants respond to virus infections by activation of RNA-based silencing, which limits infection at both the single-cell and system levels. Viruses encode RNA silencing suppressor proteins that interfere with this response. Wild-type Arabidopsis thaliana is immune to silencing suppressor (HC-Pro)-deficient Turnip mosaic virus, but immunity was lost in the absence of DICER-LIKE proteins DCL4 and DCL2. Systematic analysis of susceptibility and small RNA formation in Arabidopsis mutants lacking combinations of RNA-dependent RNA polymerase (RDR) and DCL proteins revealed that the vast majority of virusderived small interfering RNAs (siRNAs) were dependent on DCL4 and RDR1, although full antiviral defense also required DCL2 and RDR6. Among the DCLs, DCL4 was sufficient for antiviral silencing in inoculated leaves, but DCL2 and DCL4 were both involved in silencing in systemic tissues (inflorescences). Basal levels of antiviral RNA silencing and siRNA biogenesis were detected in mutants lacking RDR1, RDR2, and RDR6, indicating an alternate route to form double-stranded RNA that does not depend on the three previously characterized RDR proteins.

Original languageEnglish (US)
Pages (from-to)481-496
Number of pages16
JournalPlant Cell
Volume22
Issue number2
DOIs
StatePublished - Feb 1 2010

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RNA-directed RNA polymerase
antiviral proteins
Turnip mosaic virus
RNA Replicase
Mosaic Viruses
Brassica napus
Virus Diseases
small interfering RNA
Arabidopsis
Small Interfering RNA
Antiviral Agents
RNA Interference
RNA interference
infection
Proteins
proteins
RNA
Virus Activation
Inflorescence
viruses

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during Turnip mosaic virus infection. / Garcia-Ruiz, Hernan; Takeda, Atsushi; Chapman, Elisabeth J.; Sullivan, Christopher M.; Fahlgren, Noah; Brempelis, Katherine J.; Carrington, James C.

In: Plant Cell, Vol. 22, No. 2, 01.02.2010, p. 481-496.

Research output: Contribution to journalArticle

Garcia-Ruiz, Hernan ; Takeda, Atsushi ; Chapman, Elisabeth J. ; Sullivan, Christopher M. ; Fahlgren, Noah ; Brempelis, Katherine J. ; Carrington, James C. / Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during Turnip mosaic virus infection. In: Plant Cell. 2010 ; Vol. 22, No. 2. pp. 481-496.
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