Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Karsten Klopffleisch, Nguyen Phan, Kelsey Augustin, Robert S. Bayne, Katherine S. Booker, Jose R. Botella, Nicholas C. Carpita, Tyrell Carr, Jin Gui Chen, Thomas Ryan Cooke, Arwen Frick-Cheng, Erin J. Friedman, Brandon Fulk, Michael G. Hahn, Kun Jiang, Lucia Jorda, Lydia Kruppe, Chenggang Liu, Justine Lorek, Maureen C. McCannAntonio Molina, Etsuko N. Moriyama, M. Shahid Mukhtar, Yashwanti Mudgil, Sivakumar Pattathil, John Schwarz, Steven Seta, Matthew Tan, Ulrike Temp, Yuri Trusov, Daisuke Urano, Bastian Welter, Jing Yang, Ralph Panstruga, Joachim F. Uhrig, Alan M. Jones

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

The heterotrimeric G-protein complex is minimally composed of G ±, G 2, and G 3 subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.

Original languageEnglish (US)
Article number532
JournalMolecular Systems Biology
Volume7
DOIs
StatePublished - Oct 6 2011

Fingerprint

Arabidopsis Proteins
Arabidopsis
G Protein
Morphogenesis
Cell Wall
Carbohydrates
G-proteins
GTP-Binding Proteins
morphogenesis
Cells
cell walls
carbohydrates
Proteins
Heterotrimeric GTP-Binding Proteins
Protein
Plant Proteins
proteins
Cell membranes
G-Protein-Coupled Receptors
scaffolding proteins

Keywords

  • AGB1
  • Arabidopsis
  • GPA1
  • RGS1
  • heterotrimeric G-proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

Klopffleisch, K., Phan, N., Augustin, K., Bayne, R. S., Booker, K. S., Botella, J. R., ... Jones, A. M. (2011). Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. Molecular Systems Biology, 7, [532]. https://doi.org/10.1038/msb.2011.66

Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. / Klopffleisch, Karsten; Phan, Nguyen; Augustin, Kelsey; Bayne, Robert S.; Booker, Katherine S.; Botella, Jose R.; Carpita, Nicholas C.; Carr, Tyrell; Chen, Jin Gui; Cooke, Thomas Ryan; Frick-Cheng, Arwen; Friedman, Erin J.; Fulk, Brandon; Hahn, Michael G.; Jiang, Kun; Jorda, Lucia; Kruppe, Lydia; Liu, Chenggang; Lorek, Justine; McCann, Maureen C.; Molina, Antonio; Moriyama, Etsuko N.; Mukhtar, M. Shahid; Mudgil, Yashwanti; Pattathil, Sivakumar; Schwarz, John; Seta, Steven; Tan, Matthew; Temp, Ulrike; Trusov, Yuri; Urano, Daisuke; Welter, Bastian; Yang, Jing; Panstruga, Ralph; Uhrig, Joachim F.; Jones, Alan M.

In: Molecular Systems Biology, Vol. 7, 532, 06.10.2011.

Research output: Contribution to journalArticle

Klopffleisch, K, Phan, N, Augustin, K, Bayne, RS, Booker, KS, Botella, JR, Carpita, NC, Carr, T, Chen, JG, Cooke, TR, Frick-Cheng, A, Friedman, EJ, Fulk, B, Hahn, MG, Jiang, K, Jorda, L, Kruppe, L, Liu, C, Lorek, J, McCann, MC, Molina, A, Moriyama, EN, Mukhtar, MS, Mudgil, Y, Pattathil, S, Schwarz, J, Seta, S, Tan, M, Temp, U, Trusov, Y, Urano, D, Welter, B, Yang, J, Panstruga, R, Uhrig, JF & Jones, AM 2011, 'Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis', Molecular Systems Biology, vol. 7, 532. https://doi.org/10.1038/msb.2011.66
Klopffleisch, Karsten ; Phan, Nguyen ; Augustin, Kelsey ; Bayne, Robert S. ; Booker, Katherine S. ; Botella, Jose R. ; Carpita, Nicholas C. ; Carr, Tyrell ; Chen, Jin Gui ; Cooke, Thomas Ryan ; Frick-Cheng, Arwen ; Friedman, Erin J. ; Fulk, Brandon ; Hahn, Michael G. ; Jiang, Kun ; Jorda, Lucia ; Kruppe, Lydia ; Liu, Chenggang ; Lorek, Justine ; McCann, Maureen C. ; Molina, Antonio ; Moriyama, Etsuko N. ; Mukhtar, M. Shahid ; Mudgil, Yashwanti ; Pattathil, Sivakumar ; Schwarz, John ; Seta, Steven ; Tan, Matthew ; Temp, Ulrike ; Trusov, Yuri ; Urano, Daisuke ; Welter, Bastian ; Yang, Jing ; Panstruga, Ralph ; Uhrig, Joachim F. ; Jones, Alan M. / Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. In: Molecular Systems Biology. 2011 ; Vol. 7.
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