Arabidopsis cell wall proteome defined using multidimensional protein identification technology

Emmanuelle M. Bayer, Andrew R. Bottrill, John Walshaw, Marielle Vigouroux, Mike J. Naldrett, Carole L. Thomas, Andrew J. Maule

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

With the completion of the sequencing of the Arabidopsis genome and the recent advances in proteomic technology, the identification of proteins from highly complex mixtures is now possible. Rather than using gel electrophoresis and peptide mass fingerprinting, we have used multidimensional protein identification technology (MudPIT) to analyse the 'tightly-bound' proteome for purified cell walls from Arabidospis cell suspension cultures. Using bioinformatics for the prediction of signal peptides for targeting to the secretory pathway and for the absence of ER retention signal, 89 proteins were selected as potential extracellular proteins. Only 33% of these were identified in previous proteomic analyses of Arabidopsis cell walls. A functional classification revealed that a large proportion of the proteins were enzymes, notably carbohydrate active enzymes, peroxidases and proteases. Comparison of all the published proteomic analyses for the Arabidopsis cell wall identified 268 non-redundant genes encoding wall proteins. Sixty of these (22%) were derived from our analysis of tightly-bound wall proteins.

Original languageEnglish (US)
Pages (from-to)301-311
Number of pages11
JournalProteomics
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2006

Fingerprint

Proteome
Arabidopsis
Cell Wall
Cells
Technology
Proteins
Proteomics
Peroxidases
Gene encoding
Peptide Mapping
Secretory Pathway
Enzymes
Bioinformatics
Protein Sorting Signals
Computational Biology
Electrophoresis
Complex Mixtures
Cell culture
Suspensions
Peptide Hydrolases

Keywords

  • Arabidopsis
  • Cell wall proteome
  • Multidimensional protein identification technology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bayer, E. M., Bottrill, A. R., Walshaw, J., Vigouroux, M., Naldrett, M. J., Thomas, C. L., & Maule, A. J. (2006). Arabidopsis cell wall proteome defined using multidimensional protein identification technology. Proteomics, 6(1), 301-311. https://doi.org/10.1002/pmic.200500046

Arabidopsis cell wall proteome defined using multidimensional protein identification technology. / Bayer, Emmanuelle M.; Bottrill, Andrew R.; Walshaw, John; Vigouroux, Marielle; Naldrett, Mike J.; Thomas, Carole L.; Maule, Andrew J.

In: Proteomics, Vol. 6, No. 1, 01.01.2006, p. 301-311.

Research output: Contribution to journalArticle

Bayer, EM, Bottrill, AR, Walshaw, J, Vigouroux, M, Naldrett, MJ, Thomas, CL & Maule, AJ 2006, 'Arabidopsis cell wall proteome defined using multidimensional protein identification technology', Proteomics, vol. 6, no. 1, pp. 301-311. https://doi.org/10.1002/pmic.200500046
Bayer, Emmanuelle M. ; Bottrill, Andrew R. ; Walshaw, John ; Vigouroux, Marielle ; Naldrett, Mike J. ; Thomas, Carole L. ; Maule, Andrew J. / Arabidopsis cell wall proteome defined using multidimensional protein identification technology. In: Proteomics. 2006 ; Vol. 6, No. 1. pp. 301-311.
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