Arabidopsis AtSPL14, a plant-specific SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1

Julie M. Stone, Xinwen Liang, Emily R. Nekl, Justin J. Stiers

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The recessive Arabidopsis thaliana fumonisin B1-resistant (fbr6) mutant was identified by its ability to survive in the presence of a programmed cell death (PCD)-inducing fungal toxin FB1. The fbr6 mutant also displays altered plant architecture in the absence of FB1, most notably elongated petioles and enhanced leaf margin serration. These phenotypes are a result of a T-DNA insertion in the SQUAMOSA promoter binding protein (SBP) domain gene, AtSPL14. AtSPL14 encodes a plant-specific protein with features characteristic of a transcriptional regulator, including a nuclear localization signal sequence, a plant-specific DNA binding domain (the SBP box), and a protein interaction motif (ankyrin repeats). A transiently expressed fusion of the AtSPL14 protein to green fluorescent protein is directed to the plant nucleus. DNA sequences immediately upstream of the translation start site direct expression of the β-glucuronidase reporter gene primarily in the vascular tissues, consistent with the phenotypes of the fbr6 mutant. AtSPL14 activates transcription in yeast, with a transactivation domain residing within the N-terminal region of the protein. Recombinant AtSPL14 protein binds A. thaliana genomic DNA in vitro in the absence of other proteins. These results indicate that FBR6/SPL14 functions as a transcriptional regulator that plays a role not only in sensitivity to FB1, but also in the development of normal plant architecture.

Original languageEnglish (US)
Pages (from-to)744-754
Number of pages11
JournalPlant Journal
Volume41
Issue number5
DOIs
StatePublished - Mar 1 2005

Fingerprint

fumonisin B1
Plant Development
Arabidopsis
plant development
binding proteins
Carrier Proteins
Transcription Factors
transcription factors
promoter regions
Ankyrin Repeat
Plant DNA
Protein Interaction Domains and Motifs
Phenotype
plant architecture
Nuclear Localization Signals
Plant Proteins
Proteins
proteins
Mycotoxins
Glucuronidase

Keywords

  • Arabidopsis thaliana
  • Fumonisin b1
  • Programmed cell death
  • SBP domain
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Arabidopsis AtSPL14, a plant-specific SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1. / Stone, Julie M.; Liang, Xinwen; Nekl, Emily R.; Stiers, Justin J.

In: Plant Journal, Vol. 41, No. 5, 01.03.2005, p. 744-754.

Research output: Contribution to journalArticle

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