Arabidopsis accelerated cell death 11, ACD11, Is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels

Dhirendra K. Simanshu, Xiuhong Zhai, David Munch, Daniel Hofius, Jonathan E. Markham, Jacek Bielawski, Alicja Bielawska, Lucy Malinina, Julian G. Molotkovsky, John W. Mundy, Dinshaw J. Patel, Rhoderick E. Brown

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The accelerated cell death 11 (acd11) mutant of Arabidopsis provides a genetic model for studying immune response activation and localized cellular suicide that halt pathogen spread during infection in plants. Here, we elucidate ACD11 structure and function and show that acd11 disruption dramatically alters the invivo balance of sphingolipid mediators that regulate eukaryotic-programmed cell death. In acd11 mutants, normally low ceramide-1-phosphate (C1P) levels become elevated, but the relatively abundant cell death inducer phytoceramide rises acutely. ACD11 exhibits selective intermembrane transfer of C1P and phyto-C1P. Crystal structures establish C1P binding via a surface-localized, phosphate headgroup recognition center connected to an interior hydrophobic pocket that adaptively ensheaths lipid chains via a cleft-like gating mechanism. Point mutation mapping confirms functional involvement of binding site residues. A π helix (π bulge) near the lipid binding cleft distinguishes apo-ACD11 from other GLTP folds. The global two-layer, α-helically dominated, "sandwich" topology displaying C1P-selective binding identifies ACD11 as the plant prototype of a GLTP fold subfamily.

Original languageEnglish (US)
Pages (from-to)388-399
Number of pages12
JournalCell Reports
Volume6
Issue number2
DOIs
StatePublished - Jan 10 2014

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Cell death
Arabidopsis
Cell Death
Proteins
Lipids
Sphingolipids
Genetic Models
Eukaryotic Cells
Pathogens
Point Mutation
Suicide
Crystal structure
Chemical activation
Phosphates
Binding Sites
Topology
ceramide 1-phosphate
Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Arabidopsis accelerated cell death 11, ACD11, Is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels. / Simanshu, Dhirendra K.; Zhai, Xiuhong; Munch, David; Hofius, Daniel; Markham, Jonathan E.; Bielawski, Jacek; Bielawska, Alicja; Malinina, Lucy; Molotkovsky, Julian G.; Mundy, John W.; Patel, Dinshaw J.; Brown, Rhoderick E.

In: Cell Reports, Vol. 6, No. 2, 10.01.2014, p. 388-399.

Research output: Contribution to journalArticle

Simanshu, DK, Zhai, X, Munch, D, Hofius, D, Markham, JE, Bielawski, J, Bielawska, A, Malinina, L, Molotkovsky, JG, Mundy, JW, Patel, DJ & Brown, RE 2014, 'Arabidopsis accelerated cell death 11, ACD11, Is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels', Cell Reports, vol. 6, no. 2, pp. 388-399. https://doi.org/10.1016/j.celrep.2013.12.023
Simanshu, Dhirendra K. ; Zhai, Xiuhong ; Munch, David ; Hofius, Daniel ; Markham, Jonathan E. ; Bielawski, Jacek ; Bielawska, Alicja ; Malinina, Lucy ; Molotkovsky, Julian G. ; Mundy, John W. ; Patel, Dinshaw J. ; Brown, Rhoderick E. / Arabidopsis accelerated cell death 11, ACD11, Is a ceramide-1-phosphate transfer protein and intermediary regulator of phytoceramide levels. In: Cell Reports. 2014 ; Vol. 6, No. 2. pp. 388-399.
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