Aprotinin reduces interleukin-8 production and lung neutrophil accumulation after cardiopulmonary bypass

Gary E. Hill, Roman Pohorecki, Anselmo Alonso, Stephen I. Rennard, Richard A. Robbins

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Pulmonary neutrophil entrapment and resultant oxidative injury is thought to be the primary mechanism of cardiopulmonary bypass (CPB) induced lung injury. Interleukin-8 (IL-8), a potent neutrophil chemoattractant induced by cytokines, including tumor necrosis factor-α (TNF), is found in increased concentrations in bronchial alveolar lavage fluid (BALF) in lung inflammation. Since aprotinin reduces TNF release during CPB, the effects of aprotinin on BALF IL-8 concentrations and neutrophil levels were determined after CPB in adult humans. Study patients were equally divided into a control group (n = 8, Group 1) and an aprotinin-treated group (n = 8, Group 2). In vitro neutrophil chemotaxis was done with volunteer neutrophils using three different chemoattractants: 1) N-formyl-1-methionyl-1-leucyl-1-phenylalanine (FMLP); 2) the supernatant of a human bronchial epithelial cell culture line, A549, after 24 h of TNF stimulation with or without aprotinin or N-α-tosyl- L-lysine chloromethyl ketone (TLCK) (a potent protease inhibitor), and 3) BALF. Aprotinin treatment significantly (P < 0.05) reduced post-CPB BALF IL- 8 concentrations and percentage of neutrophils. In vitro, BALF from Group 1 had significantly greater chemotactic ability when compared with Group 2. The TNF stimulated A549 cell culture supernatant had significantly (P < 0.05) greater chemotactic ability than control supernatant, while aprotinin and TLCK significantly (P < 0.05) reduced this chemotactic ability. These results demonstrate that aprotinin blunts IL-8 production and reduces neutrophil lung accumulation post-CPB.

Original languageEnglish (US)
Pages (from-to)696-700
Number of pages5
JournalAnesthesia and analgesia
Volume83
Issue number4
DOIs
StatePublished - Oct 30 1996

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Aprotinin
Cardiopulmonary Bypass
Interleukin-8
Neutrophils
Bronchoalveolar Lavage Fluid
Lung
Aptitude
Tumor Necrosis Factor-alpha
Chemotactic Factors
Lysine
leucyl-phenylalanine
Cell Culture Techniques
Lung Injury
Chemotaxis
Protease Inhibitors
Volunteers
Pneumonia
Epithelial Cells
Cytokines
Cell Line

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Aprotinin reduces interleukin-8 production and lung neutrophil accumulation after cardiopulmonary bypass. / Hill, Gary E.; Pohorecki, Roman; Alonso, Anselmo; Rennard, Stephen I.; Robbins, Richard A.

In: Anesthesia and analgesia, Vol. 83, No. 4, 30.10.1996, p. 696-700.

Research output: Contribution to journalArticle

Hill, Gary E. ; Pohorecki, Roman ; Alonso, Anselmo ; Rennard, Stephen I. ; Robbins, Richard A. / Aprotinin reduces interleukin-8 production and lung neutrophil accumulation after cardiopulmonary bypass. In: Anesthesia and analgesia. 1996 ; Vol. 83, No. 4. pp. 696-700.
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