Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue

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Abstract

A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.

Original languageEnglish (US)
Pages (from-to)3395-3397
Number of pages3
JournalJournal of clinical microbiology
Volume50
Issue number10
DOIs
StatePublished - Oct 1 2012

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Histoplasma
Paraffin
Formaldehyde
Real-Time Polymerase Chain Reaction
Fungal DNA
Histoplasmosis

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

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abstract = "A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100{\%}, and clinical sensitivity of 88.9{\%}. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.",
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