Applications of stripe assay in the study of CXCL12-mediated neural progenitor cell migration and polarization

Min Zhang, Aihong Song, Siqiang Lai, Lisha Qiu, Yunlong Huang, Qiang Chen, Bing Zhu, Dongsheng Xu, Jialin C Zheng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The polarization and migration of neural progenitor cells (NPCs) are critical for embryonic brain development and neurogenesis after brain injury. Although stromal-derived factor-1α (SDF-1α, CXCL12) and its receptor CXCR4 are well-known to mediate the migration of NPCs in the developing brain, the dynamic cellular processes and structure-related molecular events remain elusive. Transwell and microfluidic-based assays are classical assays to effectively study cellular migration. However, both of them have limitations in the analysis of a single cell. In this study, we modified the stripe assay and extended its applications in the study of NPC polarization and intracellular molecular events associated with CXCL12-mediated migration. In response to localized CXCL12, NPCs formed lamellipodia in the stripe assay. Furthermore, CXCR4 and Rac1 quickly re-distributed to the area of lamellipodia, indicating their roles in NPC polarization upon CXCL12 stimulation. Although the chemokine stripes in the assay provided concentration gradients that can be best used to study cellular polarization and migration through immunocytochemistry, they can also generate live imaging data with comparable quality. In conclusion, stripe assay is a visual, dynamic and economical tool to study cellular mobility and its related molecule mechanisms.

Original languageEnglish (US)
Pages (from-to)163-171
Number of pages9
JournalBiomaterials
Volume72
DOIs
StatePublished - Dec 1 2015

Fingerprint

Cell Movement
Assays
Stem Cells
Polarization
Pseudopodia
Brain
Single-Cell Analysis
CXCR4 Receptors
Microfluidics
Neurogenesis
Cellular Structures
Chemokines
Brain Injuries
Embryonic Development
Immunohistochemistry
Imaging techniques
Molecules

Keywords

  • CXCL12
  • CXCR4
  • Migration
  • PDMS
  • Polarization
  • Stripe assay

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Applications of stripe assay in the study of CXCL12-mediated neural progenitor cell migration and polarization. / Zhang, Min; Song, Aihong; Lai, Siqiang; Qiu, Lisha; Huang, Yunlong; Chen, Qiang; Zhu, Bing; Xu, Dongsheng; Zheng, Jialin C.

In: Biomaterials, Vol. 72, 01.12.2015, p. 163-171.

Research output: Contribution to journalArticle

Zhang, Min ; Song, Aihong ; Lai, Siqiang ; Qiu, Lisha ; Huang, Yunlong ; Chen, Qiang ; Zhu, Bing ; Xu, Dongsheng ; Zheng, Jialin C. / Applications of stripe assay in the study of CXCL12-mediated neural progenitor cell migration and polarization. In: Biomaterials. 2015 ; Vol. 72. pp. 163-171.
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AU - Zhu, Bing

AU - Xu, Dongsheng

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