Applications of mouse airway epithelial cell culture for asthma research

Amjad Horani, John D. Dickinson, Steven L. Brody

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal epithelial cell cultures for the study of asthma biology. A major advantage of this system is the ability to use airway epithelial cells from mice with defined genetic backgrounds. The in vitro proliferation and differentiation of mouse airway epithelial cells uses the air-liquid interface condition to generate well-differentiated epithelia with characteristics of native airways. Protocols are provided for manipulation of differentiation, induction of mucous cell metaplasia, genetic modification, and cell and pathogen coculture. Assays for the assessment of gene expression, responses of cells, and analysis of specific cell subpopulations within the airway epithelium are included.

Original languageEnglish (US)
Title of host publicationMouse Models of Allergic Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages91-107
Number of pages17
ISBN (Print)9781627034951
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1032
ISSN (Print)1064-3745

Fingerprint

Asthma
Cell Culture Techniques
Epithelial Cells
Research
Epithelium
Metaplasia
Immunologic Factors
Coculture Techniques
Cell Communication
Cell Differentiation
Immune System
Air
Gene Expression

Keywords

  • Air-liquid interface
  • Asthma
  • Ciliated cell
  • Mouse
  • Mucous cell
  • Trachea

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Horani, A., Dickinson, J. D., & Brody, S. L. (2013). Applications of mouse airway epithelial cell culture for asthma research. In Mouse Models of Allergic Disease: Methods and Protocols (pp. 91-107). (Methods in Molecular Biology; Vol. 1032). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-496-8_7

Applications of mouse airway epithelial cell culture for asthma research. / Horani, Amjad; Dickinson, John D.; Brody, Steven L.

Mouse Models of Allergic Disease: Methods and Protocols. Humana Press Inc., 2013. p. 91-107 (Methods in Molecular Biology; Vol. 1032).

Research output: Chapter in Book/Report/Conference proceedingChapter

Horani, A, Dickinson, JD & Brody, SL 2013, Applications of mouse airway epithelial cell culture for asthma research. in Mouse Models of Allergic Disease: Methods and Protocols. Methods in Molecular Biology, vol. 1032, Humana Press Inc., pp. 91-107. https://doi.org/10.1007/978-1-62703-496-8_7
Horani A, Dickinson JD, Brody SL. Applications of mouse airway epithelial cell culture for asthma research. In Mouse Models of Allergic Disease: Methods and Protocols. Humana Press Inc. 2013. p. 91-107. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-496-8_7
Horani, Amjad ; Dickinson, John D. ; Brody, Steven L. / Applications of mouse airway epithelial cell culture for asthma research. Mouse Models of Allergic Disease: Methods and Protocols. Humana Press Inc., 2013. pp. 91-107 (Methods in Molecular Biology).
@inbook{cb5d4d96b322469e8ab03dcc5dc9206e,
title = "Applications of mouse airway epithelial cell culture for asthma research",
abstract = "Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal epithelial cell cultures for the study of asthma biology. A major advantage of this system is the ability to use airway epithelial cells from mice with defined genetic backgrounds. The in vitro proliferation and differentiation of mouse airway epithelial cells uses the air-liquid interface condition to generate well-differentiated epithelia with characteristics of native airways. Protocols are provided for manipulation of differentiation, induction of mucous cell metaplasia, genetic modification, and cell and pathogen coculture. Assays for the assessment of gene expression, responses of cells, and analysis of specific cell subpopulations within the airway epithelium are included.",
keywords = "Air-liquid interface, Asthma, Ciliated cell, Mouse, Mucous cell, Trachea",
author = "Amjad Horani and Dickinson, {John D.} and Brody, {Steven L.}",
year = "2013",
doi = "10.1007/978-1-62703-496-8_7",
language = "English (US)",
isbn = "9781627034951",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "91--107",
booktitle = "Mouse Models of Allergic Disease",

}

TY - CHAP

T1 - Applications of mouse airway epithelial cell culture for asthma research

AU - Horani, Amjad

AU - Dickinson, John D.

AU - Brody, Steven L.

PY - 2013

Y1 - 2013

N2 - Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal epithelial cell cultures for the study of asthma biology. A major advantage of this system is the ability to use airway epithelial cells from mice with defined genetic backgrounds. The in vitro proliferation and differentiation of mouse airway epithelial cells uses the air-liquid interface condition to generate well-differentiated epithelia with characteristics of native airways. Protocols are provided for manipulation of differentiation, induction of mucous cell metaplasia, genetic modification, and cell and pathogen coculture. Assays for the assessment of gene expression, responses of cells, and analysis of specific cell subpopulations within the airway epithelium are included.

AB - Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal epithelial cell cultures for the study of asthma biology. A major advantage of this system is the ability to use airway epithelial cells from mice with defined genetic backgrounds. The in vitro proliferation and differentiation of mouse airway epithelial cells uses the air-liquid interface condition to generate well-differentiated epithelia with characteristics of native airways. Protocols are provided for manipulation of differentiation, induction of mucous cell metaplasia, genetic modification, and cell and pathogen coculture. Assays for the assessment of gene expression, responses of cells, and analysis of specific cell subpopulations within the airway epithelium are included.

KW - Air-liquid interface

KW - Asthma

KW - Ciliated cell

KW - Mouse

KW - Mucous cell

KW - Trachea

UR - http://www.scopus.com/inward/record.url?scp=84922786141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922786141&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-496-8_7

DO - 10.1007/978-1-62703-496-8_7

M3 - Chapter

C2 - 23943446

AN - SCOPUS:84922786141

SN - 9781627034951

T3 - Methods in Molecular Biology

SP - 91

EP - 107

BT - Mouse Models of Allergic Disease

PB - Humana Press Inc.

ER -