Application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embedded tissues

D. G. Rogers

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.

Original languageEnglish (US)
Pages (from-to)762-766
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number8
DOIs
StatePublished - Jan 1 1985

Fingerprint

Enterotoxigenic Escherichia coli
Paraffin
Peroxidase
Methanol
Swine
K99 antigen

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

@article{02f8cdbdfe2b4d6187fbe5d999fa477e,
title = "Application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embedded tissues",
abstract = "The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.",
author = "Rogers, {D. G.}",
year = "1985",
month = "1",
day = "1",
doi = "10.1177/33.8.2862183",
language = "English (US)",
volume = "33",
pages = "762--766",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "8",

}

TY - JOUR

T1 - Application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embedded tissues

AU - Rogers, D. G.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.

AB - The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.

UR - http://www.scopus.com/inward/record.url?scp=0022363847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022363847&partnerID=8YFLogxK

U2 - 10.1177/33.8.2862183

DO - 10.1177/33.8.2862183

M3 - Article

C2 - 2862183

AN - SCOPUS:0022363847

VL - 33

SP - 762

EP - 766

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 8

ER -