Abstract
In recent years spin trapping techniques have been used extensively to better understand the free radical biology of phagocytic cells. These results demonstrate that spin trapping is of adequate sensitivity to detect superoxide and/or hydroxyl radical generated by these cells, and that spin trapping is capable of measuring phagosomal radicals as well. However. neither neutrophils. monocytes. nor monocyte derived macro-phages generate hydroxyl radical in the absence of exogenous iron. Furthermore. neutrophil lactoferrin and myeloperoxidase limit the magnitude (and in the case of lactoferrin the duration) of hydroxyl radical formed by neutrophils in an iron catalyzed system. Since monocytic phagocytes posxss no lactoferrin, and limited myeloperoxidase, hydroxyl radical may play an important role in the inflammatory behavior of mononuclear phagocytes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-25 |
| Number of pages | 9 |
| Journal | Free Radical Research |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1991 |
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Keywords
- Hydroxyl radical
- Monocytes
- Neutrophils
- Phagocytes
- Spin-trapping
- Superoxide
ASJC Scopus subject areas
- Biochemistry
Cite this
Application of spin trapping to human phagocytic cells : Insight into conditions for formation and limitation of hydroxyl radical. / Cohen, Myron S.; Britigan, Bradley E.; Pou, Sovitj; Rosen, Gerald M.
In: Free Radical Research, Vol. 12, No. 1, 01.01.1991, p. 17-25.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of spin trapping to human phagocytic cells
T2 - Insight into conditions for formation and limitation of hydroxyl radical
AU - Cohen, Myron S.
AU - Britigan, Bradley E.
AU - Pou, Sovitj
AU - Rosen, Gerald M.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - In recent years spin trapping techniques have been used extensively to better understand the free radical biology of phagocytic cells. These results demonstrate that spin trapping is of adequate sensitivity to detect superoxide and/or hydroxyl radical generated by these cells, and that spin trapping is capable of measuring phagosomal radicals as well. However. neither neutrophils. monocytes. nor monocyte derived macro-phages generate hydroxyl radical in the absence of exogenous iron. Furthermore. neutrophil lactoferrin and myeloperoxidase limit the magnitude (and in the case of lactoferrin the duration) of hydroxyl radical formed by neutrophils in an iron catalyzed system. Since monocytic phagocytes posxss no lactoferrin, and limited myeloperoxidase, hydroxyl radical may play an important role in the inflammatory behavior of mononuclear phagocytes.
AB - In recent years spin trapping techniques have been used extensively to better understand the free radical biology of phagocytic cells. These results demonstrate that spin trapping is of adequate sensitivity to detect superoxide and/or hydroxyl radical generated by these cells, and that spin trapping is capable of measuring phagosomal radicals as well. However. neither neutrophils. monocytes. nor monocyte derived macro-phages generate hydroxyl radical in the absence of exogenous iron. Furthermore. neutrophil lactoferrin and myeloperoxidase limit the magnitude (and in the case of lactoferrin the duration) of hydroxyl radical formed by neutrophils in an iron catalyzed system. Since monocytic phagocytes posxss no lactoferrin, and limited myeloperoxidase, hydroxyl radical may play an important role in the inflammatory behavior of mononuclear phagocytes.
KW - Hydroxyl radical
KW - Monocytes
KW - Neutrophils
KW - Phagocytes
KW - Spin-trapping
KW - Superoxide
UR - http://www.scopus.com/inward/record.url?scp=0026054083&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026054083&partnerID=8YFLogxK
U2 - 10.3109/10715769109145763
DO - 10.3109/10715769109145763
M3 - Article
C2 - 1649085
AN - SCOPUS:0026054083
VL - 12
SP - 17
EP - 25
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 1
ER -