Application of silica support in affinity chromatography

John E. Schiel, Rangan Mallik, Sony Soman, Krina S. Joseph, David S Hage

Research output: Contribution to journalReview article

69 Citations (Scopus)

Abstract

The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

Original languageEnglish (US)
Pages (from-to)719-737
Number of pages19
JournalJournal of Separation Science
Volume29
Issue number6
DOIs
StatePublished - Apr 1 2006

Fingerprint

Affinity chromatography
Affinity Chromatography
Silicon Dioxide
Silica
Ligands
Biological systems
Assays
High Pressure Liquid Chromatography

Keywords

  • Affinity chromatography
  • Biointeraction studies
  • Chiral separations
  • High-performance affinity chromatography
  • Silica

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Application of silica support in affinity chromatography. / Schiel, John E.; Mallik, Rangan; Soman, Sony; Joseph, Krina S.; Hage, David S.

In: Journal of Separation Science, Vol. 29, No. 6, 01.04.2006, p. 719-737.

Research output: Contribution to journalReview article

Schiel, John E. ; Mallik, Rangan ; Soman, Sony ; Joseph, Krina S. ; Hage, David S. / Application of silica support in affinity chromatography. In: Journal of Separation Science. 2006 ; Vol. 29, No. 6. pp. 719-737.
@article{12af711d2d43445f9d4e857e7ff817b6,
title = "Application of silica support in affinity chromatography",
abstract = "The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.",
keywords = "Affinity chromatography, Biointeraction studies, Chiral separations, High-performance affinity chromatography, Silica",
author = "Schiel, {John E.} and Rangan Mallik and Sony Soman and Joseph, {Krina S.} and Hage, {David S}",
year = "2006",
month = "4",
day = "1",
doi = "10.1002/jssc.200500501",
language = "English (US)",
volume = "29",
pages = "719--737",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Application of silica support in affinity chromatography

AU - Schiel, John E.

AU - Mallik, Rangan

AU - Soman, Sony

AU - Joseph, Krina S.

AU - Hage, David S

PY - 2006/4/1

Y1 - 2006/4/1

N2 - The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

AB - The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

KW - Affinity chromatography

KW - Biointeraction studies

KW - Chiral separations

KW - High-performance affinity chromatography

KW - Silica

UR - http://www.scopus.com/inward/record.url?scp=33646192500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646192500&partnerID=8YFLogxK

U2 - 10.1002/jssc.200500501

DO - 10.1002/jssc.200500501

M3 - Review article

VL - 29

SP - 719

EP - 737

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 6

ER -