Application of quantum dots to multicolor microarray experiments four-color genotyping

George Karlin-Neumann, Marina Sedova, Mat Falkowski, Zhiyong Wang, Steven Lin, Maneesh Jain

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Citations (Scopus)

Abstract

Highly multiplexed genomics assays are challenged by the need for a sufficient signal-to-noise ratio for each marker scored on a microarray-detection platform. Typically, as the number of markers scored (or target complexity) increases, either more assay-target material must be applied to the array or the specific activity of each marker must be proportionately increased. However, hybridization of excessive amounts of target to the microarray can result in elevated nonspecific binding and consequent degradation of information. We have found that quantum dots provide a successful alternative to organic dyes for achieving highly multiplexed (>20,000-plex) and highly accurate, four-color genotyping and have the additional advantage of being excitable by a single wavelength of light despite their distinct emission wavelengths.

Original languageEnglish (US)
Title of host publicationQuantum Dots
Subtitle of host publicationApplications in Biology
EditorsMarcel P. Bruchez, Charles Z. Hotz
Pages239-251
Number of pages13
DOIs
StatePublished - Feb 2 2007

Publication series

NameMethods in Molecular Biology
Volume374
ISSN (Print)1064-3745

Fingerprint

Quantum Dots
Signal-To-Noise Ratio
Genomics
Coloring Agents
Color
Light

Keywords

  • Barcodes
  • Genotyping
  • MIP
  • Microarray
  • Molecular inversion probes
  • Multicolor
  • Multiplex
  • Padlocks
  • Quantum dot
  • SNP
  • Single-nucleotide polymorphism
  • Tag arrays
  • Targeted
  • Unamplified genomic DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Karlin-Neumann, G., Sedova, M., Falkowski, M., Wang, Z., Lin, S., & Jain, M. (2007). Application of quantum dots to multicolor microarray experiments four-color genotyping. In M. P. Bruchez, & C. Z. Hotz (Eds.), Quantum Dots: Applications in Biology (pp. 239-251). (Methods in Molecular Biology; Vol. 374). https://doi.org/10.1385/1-59745-369-2:239

Application of quantum dots to multicolor microarray experiments four-color genotyping. / Karlin-Neumann, George; Sedova, Marina; Falkowski, Mat; Wang, Zhiyong; Lin, Steven; Jain, Maneesh.

Quantum Dots: Applications in Biology. ed. / Marcel P. Bruchez; Charles Z. Hotz. 2007. p. 239-251 (Methods in Molecular Biology; Vol. 374).

Research output: Chapter in Book/Report/Conference proceedingChapter

Karlin-Neumann, G, Sedova, M, Falkowski, M, Wang, Z, Lin, S & Jain, M 2007, Application of quantum dots to multicolor microarray experiments four-color genotyping. in MP Bruchez & CZ Hotz (eds), Quantum Dots: Applications in Biology. Methods in Molecular Biology, vol. 374, pp. 239-251. https://doi.org/10.1385/1-59745-369-2:239
Karlin-Neumann G, Sedova M, Falkowski M, Wang Z, Lin S, Jain M. Application of quantum dots to multicolor microarray experiments four-color genotyping. In Bruchez MP, Hotz CZ, editors, Quantum Dots: Applications in Biology. 2007. p. 239-251. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-369-2:239
Karlin-Neumann, George ; Sedova, Marina ; Falkowski, Mat ; Wang, Zhiyong ; Lin, Steven ; Jain, Maneesh. / Application of quantum dots to multicolor microarray experiments four-color genotyping. Quantum Dots: Applications in Biology. editor / Marcel P. Bruchez ; Charles Z. Hotz. 2007. pp. 239-251 (Methods in Molecular Biology).
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