Application of electrochemical surface plasmon resonance spectroscopy for characterization of electrochemical DNA sensors

S. Ehsan Salamifar, Rebecca Y. Lai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report the use of electrochemical surface plasmon resonance spectroscopy (EC-SPR) in the characterization of electrochemical DNA sensors. Three DNA probes, including a stem-loop probe and two linear probes (LP), were used in this study. Among the three sensors, the 3xLP sensor, a new sensor design with three consecutive target recognition sites, showed the largest change in SPR signal upon hybridization to T-25, a 25-base target with overhang regions that do not bind to the 3xLP probe. A detection limit of 20. nM was determined for T-25 using this sensor. Overall, this work has demonstrated the main advantage of EC-SPR, which is the ability to monitor both optical and electrochemical signals simultaneously, from sensor fabrication to target interrogation and sensor regeneration. It also alludes to the potential use of this hybrid technique to differentiate between non-specific binding and non-specific adsorption of non-complement targets onto the sensor surface.

Original languageEnglish (US)
Pages (from-to)835-839
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume122
DOIs
StatePublished - Oct 1 2014

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Surface Plasmon Resonance
Surface plasmon resonance
surface plasmon resonance
Spectrum Analysis
DNA
deoxyribonucleic acid
Spectroscopy
sensors
DNA Probes
Sensors
spectroscopy
Adsorption
Limit of Detection
Regeneration
probes
target recognition
interrogation
regeneration
stems
optical communication

Keywords

  • Alternating current voltammetry
  • Electrochemical DNA sensors
  • Electrochemical surface plasmon resonance spectroscopy
  • Linear DNA probe
  • Methylene blue
  • Stem-loop DNA probe

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

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title = "Application of electrochemical surface plasmon resonance spectroscopy for characterization of electrochemical DNA sensors",
abstract = "We report the use of electrochemical surface plasmon resonance spectroscopy (EC-SPR) in the characterization of electrochemical DNA sensors. Three DNA probes, including a stem-loop probe and two linear probes (LP), were used in this study. Among the three sensors, the 3xLP sensor, a new sensor design with three consecutive target recognition sites, showed the largest change in SPR signal upon hybridization to T-25, a 25-base target with overhang regions that do not bind to the 3xLP probe. A detection limit of 20. nM was determined for T-25 using this sensor. Overall, this work has demonstrated the main advantage of EC-SPR, which is the ability to monitor both optical and electrochemical signals simultaneously, from sensor fabrication to target interrogation and sensor regeneration. It also alludes to the potential use of this hybrid technique to differentiate between non-specific binding and non-specific adsorption of non-complement targets onto the sensor surface.",
keywords = "Alternating current voltammetry, Electrochemical DNA sensors, Electrochemical surface plasmon resonance spectroscopy, Linear DNA probe, Methylene blue, Stem-loop DNA probe",
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T1 - Application of electrochemical surface plasmon resonance spectroscopy for characterization of electrochemical DNA sensors

AU - Salamifar, S. Ehsan

AU - Lai, Rebecca Y.

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N2 - We report the use of electrochemical surface plasmon resonance spectroscopy (EC-SPR) in the characterization of electrochemical DNA sensors. Three DNA probes, including a stem-loop probe and two linear probes (LP), were used in this study. Among the three sensors, the 3xLP sensor, a new sensor design with three consecutive target recognition sites, showed the largest change in SPR signal upon hybridization to T-25, a 25-base target with overhang regions that do not bind to the 3xLP probe. A detection limit of 20. nM was determined for T-25 using this sensor. Overall, this work has demonstrated the main advantage of EC-SPR, which is the ability to monitor both optical and electrochemical signals simultaneously, from sensor fabrication to target interrogation and sensor regeneration. It also alludes to the potential use of this hybrid technique to differentiate between non-specific binding and non-specific adsorption of non-complement targets onto the sensor surface.

AB - We report the use of electrochemical surface plasmon resonance spectroscopy (EC-SPR) in the characterization of electrochemical DNA sensors. Three DNA probes, including a stem-loop probe and two linear probes (LP), were used in this study. Among the three sensors, the 3xLP sensor, a new sensor design with three consecutive target recognition sites, showed the largest change in SPR signal upon hybridization to T-25, a 25-base target with overhang regions that do not bind to the 3xLP probe. A detection limit of 20. nM was determined for T-25 using this sensor. Overall, this work has demonstrated the main advantage of EC-SPR, which is the ability to monitor both optical and electrochemical signals simultaneously, from sensor fabrication to target interrogation and sensor regeneration. It also alludes to the potential use of this hybrid technique to differentiate between non-specific binding and non-specific adsorption of non-complement targets onto the sensor surface.

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KW - Electrochemical surface plasmon resonance spectroscopy

KW - Linear DNA probe

KW - Methylene blue

KW - Stem-loop DNA probe

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