Appearance of High Affinity Receptors for Type β Transforming Growth Factor during Differentiation of Murine Embryonal Carcinoma Cells

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Recent studies have demonstrated that type β transforming growth factor (TGF-β) not only influences cell growth but also affects cell differentiation. In the present study, two different embryonal carcinoma (EC) cell lines and their differentiated cells were examined for the presence of TGF-β receptors and for their responses to this factor. F9 and PC-13 EC cells bind little, if any, TGF-β and do not appear to respond to TGF-β in monolayer or soft agar cultures. Treatment of both EC cell lines with retinoic acid leads to the appearance of irreversibly differentiated cells that begin to exhibit receptors for TGF-β by 48 h. After an additional 3–5 days, the differentiated cells express approximately 6000 high affinity receptors for TGF-β, with an apparent dissociation constant of 45 pM. In contrast to the results observed with the parental EC cells, TGF-β influences the growth of the differentiated cells cultured in both serum-free and serum-containing media. However, TGF-β, alone or in combination with other growth factors, does not induce the differentiated cells to form colonies in soft agar. The possible relationship of these results to the roles of growth factors during early mammalian development is discussed.

Original languageEnglish (US)
Pages (from-to)4386-4390
Number of pages5
JournalCancer Research
Volume47
Issue number16
StatePublished - Jan 1 1987

Fingerprint

Embryonal Carcinoma Stem Cells
Transforming Growth Factors
Agar
Intercellular Signaling Peptides and Proteins
Cell Line
Growth Factor Receptors
Serum-Free Culture Media
Growth
Tretinoin
Cell Differentiation
Cultured Cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

@article{61c7c8f7e90847549ebe607162d3f7e1,
title = "Appearance of High Affinity Receptors for Type β Transforming Growth Factor during Differentiation of Murine Embryonal Carcinoma Cells",
abstract = "Recent studies have demonstrated that type β transforming growth factor (TGF-β) not only influences cell growth but also affects cell differentiation. In the present study, two different embryonal carcinoma (EC) cell lines and their differentiated cells were examined for the presence of TGF-β receptors and for their responses to this factor. F9 and PC-13 EC cells bind little, if any, TGF-β and do not appear to respond to TGF-β in monolayer or soft agar cultures. Treatment of both EC cell lines with retinoic acid leads to the appearance of irreversibly differentiated cells that begin to exhibit receptors for TGF-β by 48 h. After an additional 3–5 days, the differentiated cells express approximately 6000 high affinity receptors for TGF-β, with an apparent dissociation constant of 45 pM. In contrast to the results observed with the parental EC cells, TGF-β influences the growth of the differentiated cells cultured in both serum-free and serum-containing media. However, TGF-β, alone or in combination with other growth factors, does not induce the differentiated cells to form colonies in soft agar. The possible relationship of these results to the roles of growth factors during early mammalian development is discussed.",
author = "Rizzino, {A Angie}",
year = "1987",
month = "1",
day = "1",
language = "English (US)",
volume = "47",
pages = "4386--4390",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "16",

}

TY - JOUR

T1 - Appearance of High Affinity Receptors for Type β Transforming Growth Factor during Differentiation of Murine Embryonal Carcinoma Cells

AU - Rizzino, A Angie

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Recent studies have demonstrated that type β transforming growth factor (TGF-β) not only influences cell growth but also affects cell differentiation. In the present study, two different embryonal carcinoma (EC) cell lines and their differentiated cells were examined for the presence of TGF-β receptors and for their responses to this factor. F9 and PC-13 EC cells bind little, if any, TGF-β and do not appear to respond to TGF-β in monolayer or soft agar cultures. Treatment of both EC cell lines with retinoic acid leads to the appearance of irreversibly differentiated cells that begin to exhibit receptors for TGF-β by 48 h. After an additional 3–5 days, the differentiated cells express approximately 6000 high affinity receptors for TGF-β, with an apparent dissociation constant of 45 pM. In contrast to the results observed with the parental EC cells, TGF-β influences the growth of the differentiated cells cultured in both serum-free and serum-containing media. However, TGF-β, alone or in combination with other growth factors, does not induce the differentiated cells to form colonies in soft agar. The possible relationship of these results to the roles of growth factors during early mammalian development is discussed.

AB - Recent studies have demonstrated that type β transforming growth factor (TGF-β) not only influences cell growth but also affects cell differentiation. In the present study, two different embryonal carcinoma (EC) cell lines and their differentiated cells were examined for the presence of TGF-β receptors and for their responses to this factor. F9 and PC-13 EC cells bind little, if any, TGF-β and do not appear to respond to TGF-β in monolayer or soft agar cultures. Treatment of both EC cell lines with retinoic acid leads to the appearance of irreversibly differentiated cells that begin to exhibit receptors for TGF-β by 48 h. After an additional 3–5 days, the differentiated cells express approximately 6000 high affinity receptors for TGF-β, with an apparent dissociation constant of 45 pM. In contrast to the results observed with the parental EC cells, TGF-β influences the growth of the differentiated cells cultured in both serum-free and serum-containing media. However, TGF-β, alone or in combination with other growth factors, does not induce the differentiated cells to form colonies in soft agar. The possible relationship of these results to the roles of growth factors during early mammalian development is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0023216277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023216277&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 4386

EP - 4390

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 16

ER -