Apparent increases in phospholipid degradation and turnover during combined treatment with protein synthesis inhibitors and adrenocorticotropin

Robert V. Farese, Mohammad A. Sabir, John S Davis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Inhibitors of protein synthesis, cycloheximide and puromycin, blocked ACTH (adrenocorticotropin)-induced increases in phospholipid mass, including phosphatidylinositol, but paradoxically increase 32P-labelling (but not [3H]glycerol-labelling) therein. Cycloheximide also provoked an initial rapid decrease in 32P-prelabelled phospholipids, followed by an increase in [32P]Pi incorporation. These effects of cycloheximide and puromycin occurred in ACTH-treated (but not in control) cells. It appears that inhibition of protein synthesis during ACTH action provokes an increase in phospholipid degradation, followed by partial resynthesis of the phospholipid head groups.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume793
Issue number2
DOIs
StatePublished - Apr 18 1984

Fingerprint

Protein Synthesis Inhibitors
Adrenocorticotropic Hormone
Phospholipids
Cycloheximide
Degradation
Puromycin
Labeling
Phosphatidylinositols
Glycerol
Proteins

Keywords

  • (Rat adrenal cell)
  • Adrenocorticotropin
  • Cycloheximide
  • Phosphatidylinositol
  • Phospholipid turnover
  • Puromycin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

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abstract = "Inhibitors of protein synthesis, cycloheximide and puromycin, blocked ACTH (adrenocorticotropin)-induced increases in phospholipid mass, including phosphatidylinositol, but paradoxically increase 32P-labelling (but not [3H]glycerol-labelling) therein. Cycloheximide also provoked an initial rapid decrease in 32P-prelabelled phospholipids, followed by an increase in [32P]Pi incorporation. These effects of cycloheximide and puromycin occurred in ACTH-treated (but not in control) cells. It appears that inhibition of protein synthesis during ACTH action provokes an increase in phospholipid degradation, followed by partial resynthesis of the phospholipid head groups.",
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T1 - Apparent increases in phospholipid degradation and turnover during combined treatment with protein synthesis inhibitors and adrenocorticotropin

AU - Farese, Robert V.

AU - Sabir, Mohammad A.

AU - Davis, John S

PY - 1984/4/18

Y1 - 1984/4/18

N2 - Inhibitors of protein synthesis, cycloheximide and puromycin, blocked ACTH (adrenocorticotropin)-induced increases in phospholipid mass, including phosphatidylinositol, but paradoxically increase 32P-labelling (but not [3H]glycerol-labelling) therein. Cycloheximide also provoked an initial rapid decrease in 32P-prelabelled phospholipids, followed by an increase in [32P]Pi incorporation. These effects of cycloheximide and puromycin occurred in ACTH-treated (but not in control) cells. It appears that inhibition of protein synthesis during ACTH action provokes an increase in phospholipid degradation, followed by partial resynthesis of the phospholipid head groups.

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KW - (Rat adrenal cell)

KW - Adrenocorticotropin

KW - Cycloheximide

KW - Phosphatidylinositol

KW - Phospholipid turnover

KW - Puromycin

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JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

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