Apolipoprotein B100 biogenesis

A complex array of intracellular mechanisms regulating folding, stability, and lipoprotein assembly

Angela C. Rutledge, Qiaozhu Su, Khosrow Adeli

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Apolipoprotein B100 (apoB) is a large amphipathic lipid-binding protein that is synthesized by hepatocytes and used to assemble and stabilize very low density lipoproteins (VLDL). It may have been derived through evolution from other lipid-associating proteins such as microsomal triglyceride transfer protein or vitellogenin. The correct folding of apoB requires assistance from chaperone proteins in co-translational lipidation, disulfide bond formation, and glycosylation. Any impairment in these processes results in co-translational targeting of the misfolded apoB molecule for proteasomal degradation. In fact, most of the regulation of apoB production is mediated by intracellular degradation. ApoB that misfolds post-translationally, perhaps as a result of oxidative stress, may be eliminated through autophagy. This review focuses on the proposed pentapartite domain structure of apoB, the role that each domain plays in the binding of lipid species and regulation of apoB synthesis, and the process of VLDL assembly. The factors involved in the recognition, ubiquitination, and proteasomal delivery of defective apoB molecules are also discussed.

Original languageEnglish (US)
Pages (from-to)251-267
Number of pages17
JournalBiochemistry and Cell Biology
Volume88
Issue number2
DOIs
StatePublished - Jan 1 2010

Fingerprint

Apolipoproteins
Lipoproteins
VLDL Lipoproteins
Lipids
Glycosylation
Vitellogenins
Degradation
Molecules
Oxidative stress
Ubiquitination
Autophagy
Apolipoproteins B
Disulfides
Hepatocytes
Carrier Proteins
Proteins
Oxidative Stress

Keywords

  • ApoB
  • ER-associated degradation
  • Lipoprotein assembly
  • Misfolding

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Apolipoprotein B100 biogenesis : A complex array of intracellular mechanisms regulating folding, stability, and lipoprotein assembly. / Rutledge, Angela C.; Su, Qiaozhu; Adeli, Khosrow.

In: Biochemistry and Cell Biology, Vol. 88, No. 2, 01.01.2010, p. 251-267.

Research output: Contribution to journalArticle

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