Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein

T. Ocampo Ocampo, S. M. Gabriel Peralta, N. Bacheller, S. Uiterwaal, A. Knapp, A. Hennen, D. L. Ochoa-Martinez, Hernan Garcia Ruiz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In addition to regulating gene expression, RNA silencing is an essential antiviral defense system in plants. Triggered by double-stranded RNA, silencing results in degradation or translational repression of target transcripts. Viruses are inducers and targets of RNA silencing. To condition susceptibility, most plant viruses encode silencing suppressors that interfere with this process, such as the Tomato spotted wilt virus (TSWV) NSs protein. The mechanism by which NSs suppresses RNA silencing and its role in viral infection and movement remain to be determined. We cloned NSs from the Hawaii isolate of TSWV and using two independent assays show for the first time that this protein restored pathogenicity and supported the formation of local infection foci by suppressor-deficient Turnip mosaic virus and Turnip crinkle virus. Demonstrating the suppression of RNA silencing directed against heterologous viruses establishes the foundation to determine the means used by NSs to block this antiviral process.

Original languageEnglish (US)
Article numbergmr.15028625
JournalGenetics and Molecular Research
Volume15
Issue number2
DOIs
StatePublished - Jun 17 2016

Fingerprint

Tospovirus
RNA Interference
Antiviral Agents
Proteins
Carmovirus
Viruses
Plant Viruses
Mosaic Viruses
Brassica napus
Double-Stranded RNA
Virus Diseases
Virulence
Gene Expression
Infection

Keywords

  • Antiviral RNA silencing
  • NSs protein
  • TSWV

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ocampo Ocampo, T., Gabriel Peralta, S. M., Bacheller, N., Uiterwaal, S., Knapp, A., Hennen, A., ... Garcia Ruiz, H. (2016). Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. Genetics and Molecular Research, 15(2), [gmr.15028625]. https://doi.org/10.4238/gmr.15028625

Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. / Ocampo Ocampo, T.; Gabriel Peralta, S. M.; Bacheller, N.; Uiterwaal, S.; Knapp, A.; Hennen, A.; Ochoa-Martinez, D. L.; Garcia Ruiz, Hernan.

In: Genetics and Molecular Research, Vol. 15, No. 2, gmr.15028625, 17.06.2016.

Research output: Contribution to journalArticle

Ocampo Ocampo, T, Gabriel Peralta, SM, Bacheller, N, Uiterwaal, S, Knapp, A, Hennen, A, Ochoa-Martinez, DL & Garcia Ruiz, H 2016, 'Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein', Genetics and Molecular Research, vol. 15, no. 2, gmr.15028625. https://doi.org/10.4238/gmr.15028625
Ocampo Ocampo T, Gabriel Peralta SM, Bacheller N, Uiterwaal S, Knapp A, Hennen A et al. Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. Genetics and Molecular Research. 2016 Jun 17;15(2). gmr.15028625. https://doi.org/10.4238/gmr.15028625
Ocampo Ocampo, T. ; Gabriel Peralta, S. M. ; Bacheller, N. ; Uiterwaal, S. ; Knapp, A. ; Hennen, A. ; Ochoa-Martinez, D. L. ; Garcia Ruiz, Hernan. / Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. In: Genetics and Molecular Research. 2016 ; Vol. 15, No. 2.
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