Antiserum against neuropeptide Y enhances the nicotine-mediated release of catecholamines from cultured rat adrenal chromaffin cells

Kotaro Shimoda, Gregory H. Shen, Ronald F. Pfeiffer, Rodney D McComb, Hsiu Ying T Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A primary culture of chromaffin cells was prepared from adult rats and the stability of cell contents, NPY and catecholamines (CAs), during the culture was studied. The responsiveness of cultured chromaffin cells to NGF or secretagogues and the possible role of NPY on the CA secretion from cultured chromaffin cells were investigated. After plating of isolated cells, there was marked decrease in the cell content of CAs but a significant increase in the cell content of NPY. Though both NPY and CAs in the cultured cells were positively regulated by NGF, the results of this study seemed to suggest a differential regulation for NPY and CAs in the chromaffin cell. The cultured chromaffin cells secreted NPY and CAs in response to stimulation by nicotine. The nicotine stimulated secretion of CA was enhanced by the presence of IgG fraction, prepared from NPY antiserum, in the secretion medium. The results suggested that NPY was co-released with CAs from chromaffin cells and then acted as a modulator on CA secretion.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalNeurochemistry International
Volume23
Issue number1
DOIs
StatePublished - Jul 1993

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Chromaffin Cells
Neuropeptide Y
Nicotine
Catecholamines
Immune Sera
Cultured Cells
Nerve Growth Factor
Immunoglobulin G

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Antiserum against neuropeptide Y enhances the nicotine-mediated release of catecholamines from cultured rat adrenal chromaffin cells. / Shimoda, Kotaro; Shen, Gregory H.; Pfeiffer, Ronald F.; McComb, Rodney D; Yang, Hsiu Ying T.

In: Neurochemistry International, Vol. 23, No. 1, 07.1993, p. 71-77.

Research output: Contribution to journalArticle

Shimoda, Kotaro ; Shen, Gregory H. ; Pfeiffer, Ronald F. ; McComb, Rodney D ; Yang, Hsiu Ying T. / Antiserum against neuropeptide Y enhances the nicotine-mediated release of catecholamines from cultured rat adrenal chromaffin cells. In: Neurochemistry International. 1993 ; Vol. 23, No. 1. pp. 71-77.
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